血管生成抗体芯片与研究麦冬多糖对内皮细胞瘦素表达影响.docVIP

血管生成抗体芯片研究麦冬多糖内皮细胞王硕1 冯怡1徐德生2 章漳1 丁侃3 上海中医药大学上海201203上海中医药大学上海20上海201203）摘要目的探讨麦冬多糖MDG-1对体外培养的微血管内皮细胞瘦素表达的影响。方法检测MDG-1处理后人内皮细胞生长因子生长因子mRNA表达。结果MDG-1对多种生长因 MDG-1对瘦素蛋白表达均有明显的抑制作用，RT-PCR结果也表明MDG-1可以抑制瘦素mRNA表达。结论MDG-1可以显著抑制人微血管内皮细胞mRNA和蛋白的表达，MDG-1治疗冠心病和糖尿病作用可能与调节瘦素表达有关。关键词麦冬多糖内皮细胞Effect of Polysaccharide from Ophiopogon japonicus on the expression of leptin in endothelial cells by using Angiogenesis Antibody Arrays WANG Shuo1, FENG Y11*, XU De-sheng2, ZHANG Zhang1, DING Kan3 (1Department of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China；2Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China；3Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China) 资助项目：上海市教委重点学科（No. J50302） 作者简介：王硕，男，博士研究生 * 通讯作者：冯怡，女，教授，博士生导师 Tel：021 E-mail: fyi@ ABSTRACT OBJECTIVE: To investigate the effect of a polysaccharide from O. japonicus (MDG-1) on the expression of leptin protein and mRNA in human microvascular endothelial cells (HMEC-1) by using Angiogenesis Antibody Arrays. METHODS: To find out the growth factors relative with the treatment of MDG-1 on coronary heart disease and diabetes, the expressions of 19 cytokines were evaluated using Angiogenesis Antibody Arrays with 0.2、1、10 mmol/L of MDG-1 in HMEC-1 cells. Then, the expression of leptin, the growth factor determined by Angiogenesis Antibody Arrays, mRNA was detected by RT-PCR．R After treated with MDG-1 the expression of leptin protein and mRNA in HMEC-1cells was significantly suppressed in comparison with control group (P 0.01)．CMDG-1 could significantly suppress the expression of leptin protein and mRNA in HMEC-1cells and leptin might play a role in the treatment of MDG-1 on coronary heart disease and diabetes．KEY WORDS: polysaccharide from Ophiopogon japonicus；HMEC-1；leptin；Angiogenesis Antibody Arrays瘦素 (1eptin) 又称“消脂素”，是肥胖基因 (obese gene) 的产物是由脂肪组织分泌的一种蛋白质激素，通过与其受体结合发挥抑制食欲、减少能量摄入、增加能量消耗、抑制脂肪合成、调节机体脂肪沉积的作用