HPLC法测定不同产地火麻仁药材中胡芦巴碱的含量.pdfVIP

化 学 工 程 师 Sum226No.7 Chemical Engineer 2014年第07期 文章编号：1002-1124（2014 ）07-0029-03 分 析 测 试 蔡明宸，张北灿，鲁雅文，夏林波，朱 江，邓仕任 （辽宁中医药大学药学院，辽宁大连116600） 摘 要：目的：建立高效液相色谱测定火麻仁药材中胡芦巴碱含量的方法，为2015版药典火麻仁指标性 成分的含量测定项提供依据。方法：采用Hypersil氨基柱，流动相乙腈－水（80 ∶20，V ∶V），检测波长265nm。 结果：胡芦巴碱进样量在0.104~1.04μg范围内线性关系良好，r=0.9999（n=6），加样回收率100.7%，RSD=1.56% -1 （n=9）；10批火麻仁药材中胡芦巴碱的含量为0.6504～0.99 1mg·g 。结论：胡芦巴碱为火麻仁药材及饮片中 的特征成分，其提取及含测方法操作简便，结果可靠，可作为火麻仁药材质量控制的指标性成分。 关键词：火麻仁；胡芦巴碱；高效液相色谱法；中国药典 中图分类号：O657.7 文献标识码：A Determination of trigonelline in fruit of Cannabis sativa from different origin by HPLC* CAI Ming-chen ，ZHANG Bei-can ，LU Ya-wen ，XIA Lin-bo ，ZHI Jiang ，DENG Shi-ren （College of Medicine, Liaoning Uniuersity of Traditional Cheinese Medicine, Dalian 116600, China ） Abstract ：Objective: An HPLC method for determination of trigonelline in fruit of Cannabis sativa was devel－ oped, to provide a quality control method for 2015 edition of Chinese Pharmacopoeia. Methods: The chromatogra － phy was carried out on a Hypersil amino column （250mm×4.6mm, 5μm ）with acetonitrile- water （80:20, V:V）as the mobile phase, the detection wavelength was set at 265nm. Results: Trigonelline could be successfully deter － mined and the linear rang was 0.104~1.04 μg （r=0.9999 ）. The average recoveries （n = 9 ）of the trigonelline was 100.7% ，RSD =1.56% . The content of trigonelline in 10 batches was 0.6504 ~0.9931mg ·g -1. Conclusion: Trigonelline was indeed the specific ingredient in fruit and seed of Cannabis sativa. The method is accurate, reli － abl