阶梯缓释抗结核纳米人工骨复合体的生物安全性研究.pdfVIP

窑论 著 窑 中国医药导报 2014年6月第 11卷第 18期 阶梯缓释抗结核纳米人工骨复合体的 生物安全性研究 席焱海 何海龙 李 坤 刘希麟 薛敏涛 徐 宁 叶晓健银 第二军医大学附属长征医院骨科医院脊柱外科袁上海 200003 [摘要] 目的研究阶梯缓释抗结核纳米人工骨复合体渊茁-TCP/INH-RFP-PCL/SM-SA冤的生物安全性遥 方法对 茁- TCP/INH-RFP-PCL/SM-SA复合体进行体外噻唑蓝渊MTT冤细胞毒性实验袁动物体内急性全身毒性实验尧原发性皮 肤刺激实验尧 皮内刺激实验和致敏实验袁 并设置对照组进行比较遥 结果 茁-TCP/INH-RFP-PCL/SM-SA复合体 MTT细胞毒性实验显示人工骨材料组细胞存活率在95%以上袁细胞毒性 1级遥 人工骨复合材料浸取液不引起豚 鼠过敏遥原发性皮肤刺激实验和皮内刺激实验均提示复合材料对兔皮肤尧皮内无刺激反应遥小鼠尾静脉注射后未 出现明显全身毒性反应遥 结论 茁-TCP/INH-RFP-PCL/SM-SA无明显细胞毒性袁具有良好的细胞相容性袁在应用 过程中能够降低直接使用抗结核药物所带来的生物毒性遥 动物全身毒性反应尧皮肤/内刺激反应和急性过敏反应 提示该材料具有良好的生物安全性遥 [关键词] 结核曰药物缓释曰纳米曰生物安全性 [中图分类号] R943 [文献标识码]A [文章编号] 1673-7210渊2014冤06渊c冤-0022-05 Biological security of ladder-sustained-release anti-TB drugs artificial compositebone XI Yanhai HEHailong LI Kun LIUXilin XUE Mintao XU Ning YEXiaojian银 Department of Spinal Surgery, Orthopaedic Hospital of Shanghai Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China [Abstract] Objective To investigate the biological security of ladder-sustained-release anti-TB drugs artificial com鄄 posite bone (茁-TCP/INH-RFP-PCL/SM-SA). Methods MTT toxicity test, acute systemic toxicity test, intracutaneous irritation test and skin sensitization in stimulationwere performed inthe ladder-sustained-release anti-TB drugs artifi鄄 cial composite bone. The results were compared with control group. Results MTT toxicity test showed that the survival rate of bone cells in artificial materials was more than 95% and cytotoxicity was on grade 1.Artificial bone composite material liquid did not cause allergic reaction in guinea pig. Primary skin irritation experiments and intradermal stimu鄄 lation experiment suggestedthat composite materials to rabbit skin and in