呼吸重症监护病房多重耐药鲍曼不动杆菌耐药基因检测及耐药性分析.pdfVIP

m 沦 ¨靴呼 吸重症监护病房多重耐药鲍曼不动杆菌耐药 基因检测及耐药性分析 陈炎添，熊 燕，苏雪棠，谭雪萍，廖隆祥，杨慧敏，陈青龙 (江门市人民医院检验科，广东江门529020) 摘要：目的 了解呼吸重症监护病房内多重耐药鲍曼不动杆茵(MDR-AB)伊内酰胺酶基因和耐消毒剂磺胺复 合物基因(qacE,△1-sull)的耐药性，为临床的合理用药提供依据。方法时分离出的39株MDR-AB采用聚合酶健反 (VIM-2)和产巯基变量型(SHV)均未检出。在呼吸重症监护病房分离出来的菌株对哌拉西蛛、头孢他啶、环丙沙星、 氨苄西林、头孢哌酮、头孢克洛、头孢唑啉的耐药率均90％，对多黏菌素B的敏感率为100％。结论本院呼吸重症 加强有效的监测和隔离工作。 关键词：交叉感染；呼吸监护病房；鲍氏不动杆菌；多药耐药相关蛋白质类；聚合酶链反应 中圈分类号：R974文献标识码：A文章编号：1004—583X(2012)19—1684—04 Detectionand of of resistantAcinetobacter analysisdrug-resistantgenesmulti-drug unit baumanniiin intensivecare respiratory CHENYan-tian，XIONG-Yan，SU Hui—min，CHEN Xue-tang，TANXue-ping，LIAOLong-，xiang，YANG Qing-long 529020，China DepartmentofLaboratory，．HangmenPeople’sHospital，Jiangmen To of theantimicrobia]【resistance anddisin,[ectants- ABSTRACT：01bjectiveinvestigate gene plaetamase in resistantAeinetobaeterbaumannii(MDR-AB)in sulhnilamide-resistance(qacE,△1一sull)genemulti—drug respiratory care for intensiveunit．To evidencerationalantibioticsadministration,．MethodsstrainsofMDRo provide Thirty-nine AB were related were chainreaction(PCR)andverifiedDNA separated．Thegenes analyzedbypolymerase by In strainsofMDl0AB，theratesof l。 sequencing．Resultsthirty-nine positive