去甲斑蝥素对人肺腺癌A549细胞的作用研究.pdfVIP

去甲斑蝥素对人肺腺癌 A549 细胞的作用研究 崔宝弟 王敏 孙震晓* （北京中医药大学中药学院生物制药系，北京100102） 【摘要】背景与目的：去甲斑蝥素（norcantharidin ，NCTD ）是我国自主研发的一种抗肿瘤药物， 临床上主要用于消化道肿瘤及肺癌的治疗，其抗癌机制尚待研究。本文主要研究NCTD 对人肺腺癌 A549 细胞的细胞毒作用，并初步探讨其作用机制。材料与方法：以肺癌A549 细胞为研究对象，通 过 MTT 实验，检测 NCTD 在 0-72 小时内对 A549 细胞的生长抑制作用；NCTD 处理 A549 细胞 24h 后换正常培养基，通过 MTT 实验检测细胞的恢复与增殖情况；倒置显微镜观察 A549 细胞用 NCTD 处理后细胞形态学的改变，并通过流式细胞术检测细胞的凋亡情况。结果：实验剂量范围内 NCTD 对 A549 细胞 有明显的生长抑制作用，且存在时间、剂量依赖性；NCTD 对 A549 细胞的抑制作用 在药物撤除后生长抑制作用消失较快。40µmol/L、50µmol/L 和 60µmol/L NCTD 作用 0-72h，对A549 细胞表现为明显的 G2-M 期阻滞，呈时间剂量依赖性，与对照组相比，NCTD 作用诱导 A549 细胞凋 亡作用不明显。结论：NCTD 主要通过诱导 A549 细胞 G2-M 期阻滞抑制细胞生长。 【关键词】 去甲斑蝥素；肺癌； A549 细胞； 细胞周期；细胞凋亡 中图分类号：R966 文献标识码：A Norcantharidin research on human lung adenocarcinoma A549 cells [ABSTRACT] BACKGROUND and OBJECTIVE: Norcantharidin(NCTD) is the independent research and development of a kind of antitumor drugs in our country, mainly used in the digestive tract tumor and lung cancer in clinical treatment . Its anti-cancer mechanism is yet to be studied. This paper mainly researched the effect of NCTD on lung cancer, and explored its action mechanism. MATERLAL AND METHODS: The research object was lung cancer cells A549.The growth inhibition of A549 cell treated with NCTD on a scale of 0-72 hours was detected by MTT assay. The recovery growth and proliferation of A549 cell treated with NCTD 24h was detected by MTT assay. The morphology of A549 cell treated with NCTD was detected by inverted microscope. The apoptosis effect and cycle effect of A549 cells treated with NCTD was detected by flow cytometry. RESULTS: NCTD inhibited the growth of A549 in a time and dose dependent manner within the scope of this test dose. And