Evaluation of the XE-5000 for the automated analysis of blood cells外文文献.pdf

Available online at Clinical Biochemistry 42 (2009) 684 – 691 Evaluation of the XE-5000 for the automated analysis of blood cells in cerebrospinal fluid Klas Boer ⁎, Thomas Deufel, Mike Reinhoefer Institut fuer Klinische Chemie und Laboratoriumsdiagnostik, Erlanger Allee 101, 07740 Jena, Germany Received 14 September 2008; received in revised form 6 January 2009; accepted 28 January 2009 Available online 20 February 2009 Abstract Objectives: Counting of cells in cerebrospinal fluid is an important clinical laboratory test and elevated white blood cell counts in cerebrospinal fluid are frequently seen in CNS disorders. Quantification of red blood cell concentrations in CSF may help to interpret certain diagnostic constellations and may result from subarachnoid haemorrhage, surgical procedures or contamination due to traumatic puncture. Table top analyser XE-5000 (Sysmex, Norderstedt, Germany) offers, beside its use as a haematology analyser, a protocol for the quantification of red and white blood cells in body fluids such as CSF including the differentiation between polymorphonuclear and mononuclear cells. A detection limit of 1 cell/mm3 would render this device suitable for automated CSF analysis. Design and methods: White blood cell counting was compared between Fuchs–Rosenthal counting chamber and XE-5000 in 273 routinely collected lumbar and ventricular CSF samples. Red blood cell counting was compared between UF-100 and XE-5000. Differentiation was performed on a slide stained after Pappenheim and compared to the differential count of the XE-5000. Results: Linear