化学因素抑杀菌效果评价.docVIP

中央民族大学生命与环境科学学院 微生物综合设计性实验报告 2015年1月4日 化学抑杀菌剂的效果评价 —青霉素链霉素抑菌效果评价 杜盈雪 中央民族大学 北京市海淀区 100081 摘要：目的 本实验以大肠杆菌、白色葡萄球菌为实验材料，选取青霉素、链霉素两种抗生素为化学抑菌剂，通过观察抑菌圈的大小确定抗生素的有效浓度来判断抗生素的抑菌效果，并进行同一抗生素对不同菌种抑菌效果的比较以及不同抗生素对同一菌种抑菌效果的比较。方法 本实验通过滤纸片法来确定两种抗生素对两种菌种的最小抑制细菌浓度MIC来判断抗生素的抑菌效果。并通过挑取抑菌圈内的菌种于新的培养基继续培养的方法判断抗生素的效果是抑菌还是杀菌。结果 链霉素对白色葡萄球菌的最小抑菌浓度是12μg/ml；链霉素对大肠杆菌的最小抑菌浓度是10μg/ml。青霉素对白色葡萄球菌的最小抑菌浓度为500μg/ml；青霉素对大肠杆菌的最小抑菌浓度为550 μg/ml。链霉素青霉素对大肠杆菌和白色葡萄球菌均为抑菌作用。结论 青霉素和链霉素均为作用强度中等的抗生素，链霉素的抑菌作用强于青霉素。 关键词：大肠杆菌、白色葡萄球菌、抗生素、最小抑制细菌浓度MIC，抑杀菌 Evaluation of bactericide effect of chemical inhibition —Evaluation of bactericide effect of penicillin and streptomycin Du yingxue Minzu University of China Haidian District, Beijing 100081 Abstract：Objective In this experiment, We make escherichia coli, Staphylococcus aureus as experiment materials, the selection of penicillin, streptomycin two antibiotics as chemical antimicrobial agent,in order to determine the inhibitory effect of antibiotics by observing the size of bacteriostatic circle determine the effective concentration of the antibiotics, and compared the same antibiotic bacteriostatic effect on different species and different antibiotic bacteriostatic effect on the same strain comparison . Methods This experiment by filter paper method to determine two kinds of antibiotics against two strains bacteria MIC to determine the minimum inhibitory concentration of the inhibitory effect of antibiotics. Method for judging the antibiotic and bacteriostatic ring through the selected within the strains in the new culture medium to culture effect is bacteriostatic or bactericidal. Results The minimum inhibitory concentration of streptomycin on Staphylococcus aureus is 12 μg/ml; minimum inhibitory concentration of streptomycin on Escherichia coli is 10 μg/ml. The minimum inhibitory concentrations of penicillin on white staphylococcus is 500 μg/ml; the minimum inhibitory concentrations of penicillin on Escher