小麦-中间偃麦草抗条锈病渗入系的分子细胞学鉴定.pdfVIP

，萄巍塑 OnIine 囊量生●由I掌鼙 孱／ system：http：／^^n^n^，．iabiotech．org 蹁 枷m冀麓赫赫‰韶 !… 盛乏 小麦．中间偃麦草抗条锈病渗入系的分子细胞学鉴定 詹海仙“2畅志坚2李光蓉1贾举庆3郭慧娟2张晓军2李欣2乔麟轶2杨足君卜 030801 +通讯作者，y孤gzujllIl@uestc．edu．cn 摘 要 小麦品系CH5383是源于中间偃麦草(孔i，聊yrMmime瑚edium)的兼抗多种小麦病害的新种质。 landmark 麦草DNA片段插入。用135对PLuG(PCR-basedunique 增出大约1 以作为优异的小麦抗病育种新种质加以利用。 关键词小麦，中间偃麦草，渗入系，原位杂交，PLUG标记 andMolecular Cytogenetic Identificationof aⅥmeat(乃它ic配mo∞￡如配m)- imem配击配m LineL1newithW1tn l凡己，砌p’配，n己merme口z配，nln|口O粤叮eSSlOn KeSlStanCeResis切ncetotOSmDeKUStRust 砚i聊椰m 111仃ogression Stripe zHANHai—xiaIl旧cHANGzhi—Jian2LI Hui．Juan2zHANGXiao—JuIl2 GuaIlg-Ron91JIAJu—Qin岔Guo LIXin2 Lin—Yi2YANG Q认0 Zu．Junl’ 1schoolofLifeScienceand Elec仃onicsciencearId of Instituteof TecllIl010酗u11iVers时of TecllIlology 610054，Chilla；2 china，Chcngdu Cmp +Co盯espondingauthor，yangzujun@uestc．edu．cn Abstract infer，聊以um lineCH5383is resistanceto Wheat(Zh￡icMmo∞ti秽um)一Zh讥opMmin仃ogression high wheat mst stripe and wascharacterized powde可mildew．It bygenomic讥si￡M molecularmarker DNAas nuorescence讥sitⅡhybridization(FISH)andanalysis．GISHusing死．imer玎记diMm coul血’tdetectthealien thedistal ofwheatchromosome probe signals．S仃Dng hybridizatiOnsignalin region 3BLwasobservedwhenFISH the GAA asa