稻蝗属部分种类RAPD及其分子系统学关系.pdfVIP

Acta Genetica Sinica, June 2003, 30 ( 6) : 533~ 539 ISSN 0379- 4172 Molecular Phylogenetic Relationships among Species in Oxya Serville( Orthoptera: Catantopidae) Based on Random Amplified Polymorphic DNA( RAPD) ¹ ZHANG Ji n-Zhen, MA En-Bo , GUO Y-Ping ( College of Lfi e Science and Technology , Shanxi University , Taiyuan 030006, China) Abstract: The molecul r phylogenetic rel tionships of five species of Oxya Audient-serville including O . chinensis , O . brachy- p tera, O . ag avisa, O .j ap onica nd O . intricata were studied with RAPD. Genomic DNA of forty-one individu ls were mplified with eight oligonucleotide ( 10 mer) primers which were previously selected, the specific l b nds occured in ll them, tot l of 96 cle r nd reproducible b nds ( r ng from 200~ 2500 bp) were gener ted, 95 of which were polymorphic b nd, the only one b nd ( 850 bp) which w s mplified with S362 w s common to five species of Oxya . The obt ining segments of individu l primer were between 8~ 16, the ver ge w s 12. A molecul r phylogenetic tree b sed on w s constructed Euclide n dist nce mong five species of rice gr sshopper by between-groups link ge method, the result indic ted O. chinensis w s closely rel ted to O. brachyp tera, the genetic rel tionship of O.j ap onica nd O . agavisa w s lso close, where s O. intricata h d f r phylogenetic rel tionship with them.The results of dendrogr m were consistent with the previous conclusion of morphologic cl ssific tion nd cytot xonomy, nd suggested RAPD w s suit ble for n lysis of phylogenetic rel tionships mong species of Oxya . Key ords: Oxya; RAPD; dendrogr m; phylogenetic rel tionship RAPD