紫外分光光度法测定海南木莲叶_枝中总三萜酸的含量.pdfVIP

, J urnal fAnhuiAgr.i Sci. 2010, 38( 23) : 12460- 12461, 12466 李占东 李岩 1 1 1, 2* 1, 2 1 鲍长余 , 刘冰晶 , 毕和平 , 陈光英 , 范超君 ( 1. , 571158; 2. , 571158) [目的]测定海南木莲叶和枝中总三萜酸的含 [方法]以熊果酸为对照品, 以质 分数 5%香草醛- 冰醋酸和高氯酸系统为显 色剂, 采用紫外分光光度法在546 nm 波长处测定样品的吸光度[结果]熊果酸对照品在 02~ 16 g/m l时呈良好线性关系, 回归方程 为A = 0. 154 8C + 0. 028 6, r= 0. 999 3海南木莲叶和枝中总三萜酸的含 分别为 195%和 047% [结论]该方法操作简便, 结果稳 定, 重复性好, 易于推广 海南木莲; 三萜酸; 紫外分光光度法 R 284. 2 A 0517- 6611( 2010) 23- 12460- 02 U ltraviolet Spectrophotometric Determ ination of Tritepenoidic Acid in the Leaves and Stem s ofM ang lietia ha inanens is BAO Changyu et al ( C llege f Chem istry and Chem ical Engineering, H ainan N rm alU niversity, H aik u, H ainan 571158) Abstract [ Objective] The ami was t determ ine the c ntent f the tritepen idic acid in the leaves and the stems fM anglietia hainanensis. [M ethed] U nder the exper mi ental c nditi ns including urs lic acid as the reference, 5% vanillinacetic acid and perchl ric acid as c l r sys tem, the c ntent f tr iterpen idic acid in the leaves and stem s fM anglietia hainanensis w as determ ined by ultrav i let spectr ph t metricmeth d at 546 nm. [ R esult] The calibrati n curvewas linear( r = 0. 999 3) in the range f 0. 2- 1. 6 g /m l, and the curvewasA = 0. 154 8 C + 0. 028 6, the c ntentw as 1. 95% and 0. 47% . [ C nclusi n] The resultw as stable, and had g d repeatability. T hemeth d perated c nven iently and spread easily . Key words M anglietia hainanensis; T r itepen id ic acid; U ltravi let spectr ph t m etricmeth d (M anglietia ha inanensis), H SO , , 2 4 , , , 100 m,l , 2. 5 5. 0 m l 100m l, [ 1- 5] , , , , 2 [ 6] , 2. 5. 0 m,l 5% - , 5% - 0. 2 ml 0