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The Arabidopsis bZIP1 Transcription Factor Is Involved in Sugar Signaling, Protein Networking, and DNA Binding.pdf

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The Arabidopsis bZIP1 Transcription Factor Is Involved in Sugar Signaling, Protein Networking, and DNA Binding.pdf

MolecularPlant ·Volume3 ·Number2 ·Pages361—373 ·March2010 RESEARCHARTICLE TheArabidopsisbZIP1TranscriptionFactorIs InvolvedinSugarSignaling，ProteinNetworking， andDNA Binding ShinGeneKang 一，JohnPrice。一，Pei-ChiLin ，Jong ChanHong andJyan-ChyunJang，，，’ a DepartmentofHorticultureandCropScience，TheOhioStateUniversity,Columbus，OH43210，USA b PlantBiotechnologyCenter,TheOhioStateUniversity,Columbus．OH43210，USA c DepartmentofPlantCellularandMolecularBiology,TheOhioStateUniversity,Columbus，OH43210，USA d DepartmentofBiochemistry,GyeongsangNationalUniversity．Jinju，Gyeongnam660-701，Korea ABSTRACT Sugarsignaling isamechanism thatplantsuseto integratevariousinternaIandexternaIcuestoachieve nutrienthomeostasis，mediatedevelopmentaIprograms，andarticulatestressresponses．ManybZlPtranscriptionfactors areknowntobeinvolvedinnutrientand／orstresssignaling．AnArabidopsisSl-groupbZlPgene，Atb刁P1，wasidentified asasugar-sensitiVegeneinapreviousgeneexpressionprofilingstudy(Plant(_e，16，2128—21so)．Inthisreport，weshow thattheexpressionof t6Z_，P7isrepressedbysugarsinafast，sensitive，andreversiblewa Thesugarrepressionof tb． Z”’1isa仟ectedbyaconservedsugarsignalingcomponent，hexokinase．Besidesbeingasugar-reguIatedgene，AtbZIP1can mediatesugarsignalingandaffectgeneexpression，plantgrowth，anddevelopment．Whencarbonnutrientsarelimited， gainorIossoffunctionofAtbZlP1causeschangesintheratesofearlyseedlingestablishment．Resultsofphenotypic analysesindicatethatAtbZlP1actsasanegativeregulatorofearlyseedlinggrowt h．Usinggain-andIoss-0f-function plantsina microarray analysis，two setsofputativeAtbZlP1一regulated geneshave been jdentified．Amongthem． sugar_responsivegenesarehighlyover-represented，implicatingaroleofAtbZlP1in sugar-mediatedgeneexpression． Usingyeastwto．hybrid(Y-2．H)screensandbimolecularfluorescencecomplementation(BiFC)analyses，weareableto recapitulateextensiveC／S1AtbZlPprotein interacting network in livingcells．Finally,weshow thatAtb