孕激素和米非司酮对子宫内膜癌HHUA细胞的作用_医学论文.docVIP

孕激素和米非司酮对子宫内膜癌HHUA细胞的作用_医学论文 孕激素和米非司酮对子宫内膜癌HHUA细胞的作用_医学论文 【摘要】 目的 探讨孕激素和孕激素受体拮抗剂米非司酮对人子宫内膜癌HHUA细胞株的生长抑制作用及对其表达凋亡蛋白的影响。方法 体外培养人子宫内膜癌HHUA细胞，不同浓度孕激素和米非司酮分别作用于细胞72h，绘制细胞生长曲线计算半数有效浓度（IC50）后，分为对照组、孕激素组、米非司酮组、孕激素和米非司酮联合组，以各药物的IC50干预细胞48h后，用MTT法计算细胞抑制率，免疫组化S-P法测定各组细胞bcl-2和Fas蛋白表达的变化。结果 MTT实验表明，孕激素和米非司酮均能抑制子宫内膜癌细胞增殖。孕激素半数有效浓度为100.7537μmol/L；米非司酮的半数有效浓度为39.5534μmol/L，联合用药组抑制率显著高于单独用药组。孕激素和米非司酮联合可使bcl-2表达下降，Fas表达升高（P＜0.05）。结论 孕激素和米非司酮联合应用在子宫内膜癌HHUA细胞的抗增殖和干预bcl-2和Fas蛋白的表达方面有协同作用。 【关键词】 子宫内膜肿瘤；孕酮；米非司酮；肿瘤细胞；培养 Effects of progesterone and mifepristone on the human endometrial cancer cell line HHUA 【Abstract】 Objective To study the effects of progesterone and mifepristone on the growth inhibitions and apoptosis protein of human endometrial cancer cell line.Methods HHUA cells，treated by different concentrations of progesterone and mifepristone were examined for their growth inhibitions with methyl thiazolyl tetrazolium（MTT） assay after 72 hours and got the inhibitory concentration 50%（IC50）. HHUA cells，treated by control，progesterone or mifepristone or progesterone combined mifepristone for 48 hours，were examined for their inhibitory rate with MTT and for their bcl-2 and Fas with immunohistochemistry assay.Results HHUA cells growth was inhibited by progesterone and mifepristone.IC50 of progesterone is 100.7537μmol/L；IC50 of mifepristone is 39.5534μmol/L.The inhibitory rate of progesterone combined with mifepristone was obviously stronger than that of progesterone and mifepristone. The expression of bcl-2 was decreased and Fas increased（P＜0.05）. Conclusion HHUA cells was treated by progesterone combined with mifepristone and synergistic effect was observed on the inhibitory growth and the expression of bcl-2 and Fas. 【Key words】 endometrial neoplasms；mifepristone；progesterone；tumor cells；culture 近20余年来，子宫内膜癌的发病率有上升趋势，且随着发病年龄的年轻化，保留生育能力的治疗方案已成为患者的迫切需求。激素治疗是年轻子宫内膜癌患者的主要保守治疗手段之一，研究显示［1］，年轻未育的内膜癌Ⅰ期患者，如果细胞分化好可以考虑保守治疗，同时严密监测有无复发，等待完成生育后再行手术治疗。目前子宫内膜癌激素治疗的主要药物有高效孕激素及三苯氧胺。有研究表明，孕