小叶女贞对免疫功能损伤小鼠T淋巴细胞免疫功能的调节作用_医学论文.docVIP

小叶女贞对免疫功能损伤小鼠T淋巴细胞免疫功能的调节作用_医学论文 小叶女贞对免疫功能损伤小鼠T淋巴细胞免疫功能的调节作用_医学论文 作者：韩兆东 阮月芹 宋月雁 侯佃臻 孙丰润 孔祥华 【摘要】 研究小叶女贞对免疫功能损伤小鼠T淋巴细胞免疫功能的调节作用。方法 以免疫功能损伤小鼠为研究对象，将实验鼠随机分为5组，每组12只，分别为生理盐水（NS）对照组、环磷酰胺（Cy）对照组、Cy+小叶女贞水煎液（小剂量）组、Cy+小叶女贞水煎液（大剂量）组、Cy+女贞子水煎液组。利用流式细胞仪检测实验鼠外周血T淋巴细胞（CD4+、CD8+）的百分率，计算CD4+/ CD8+比值，用全自动酶标仪检测外周血IL2的含量。结果 小叶女贞小剂量组和大剂量组CD4+T细胞、CD4+/ CD8+、IL2的含量分别与环磷酰胺组比较均明显增高，有显著性差异（P＜0.01）；与生理盐水对照组比较，小剂量组无显著性差异（P＞0.05）、大剂量组明显增高，有显著性差异（P＜0.01）；与女贞子对照组比较，小剂量组结果低，有显著性差异（P＜0.01）、大剂量组无显著性差异（P＞0.05）。结论 小叶女贞能使小鼠受损伤的免疫功能得到改善, 能提高CD4+T细胞的数量和IL2含量，对免疫功能的调节作用与剂量成正相关。小叶女贞对T淋巴细胞免疫功能的调节机制是促进T淋巴细胞分化、发育为成熟的CD4+T细胞，达到调节机体细胞免疫功能的作用。 【关键词】 小叶女贞 小鼠 免疫功能损伤 T淋巴细胞免疫功能 调节作用 【Abstract】 Objective To explore the modulation effect and mechanism of ligustrum quihoui carr(LQC) on the immune function of T lymphocytes in mouse whose immune function was damaged.Methods Mice with impaired immune function were experimental animal ,and divided into five groups randomly,twelve mice in every group,the decoction of LQC acted as experimental sample ,using the Cyclophosphamide and 0.9% NaCl as the positive comparison sample and negative comparison sample respectively, LQC minor dose,major dose,and fructus ligustri lucidi,adding Cyclophosphamide respectively,The percentage of T lymphocytes（CD4+、CD8+） in the peripheral blood of the experimental mice was detected by flow cytometer , then calculated the ratio of CD4+ to CD8+,the content of IL2 in the peripheral blood is examined by Denley.Results Contrast minor dose group and major dose group with Cyclophosphamide group respectively , the result was that the content of CD4+T cell CD4+/CD8+ IL2 all improved dramatically ,with significant difference (P＜0.01).When drawing a parallel between 0.9% NaCl and minor dose group ,there was no significant difference (P＞0.05).But when compared with the major dose group,there was significant difference between them(P＜0.01). Compared with fructus ligustri lucidi , in the minor dose group ,the curative effect was worse ,there was si