实验性变应性鼻炎与血清元素锌铜钙及细胞因子的相关性研究_药学论文.docVIP

实验性变应性鼻炎与血清元素锌铜钙及细胞因子的相关性研究_药学论文 实验性变应性鼻炎与血清元素锌铜钙及细胞因子的相关性研究_药学论文 【摘要】 　　目的通过建立肺气虚变应性鼻炎(AR)病证复合动物模型，分别检测动物体内血清元素及细胞因子水平，观察细胞因子网络Th1/Th2失衡对于AR及肺气虚证的影响，及血清元素在体内的含量变化与细胞因子的相关性。方法采用烟熏法及TDI鼻腔激发法，建立豚鼠肺气虚变应性鼻炎病证结合模型，运用原子吸收分光光度法检测致敏豚鼠血清Zn，Cu，Ca含量，运用双抗体夹心ELISA法检测致敏豚鼠血清IFN-r，IL-4水平，并对元素值和细胞因子水平之间进行相关分析。结果肺气虚变应性鼻炎豚鼠血清IL-4升高，IFN-r下降，IFN-r/IL-4比值降低，与正常对照组比较，差异显著（0.01），并见血清Zn值升高（0.05）。相关分析示Zn与IL-4呈正相关，与IFN-r/IL-4比值呈负相关，Ca与IL-4呈负相关，与IFN-r/IL-4比值呈正相关。结论AR疾病及肺气虚证均与Th1/Th2失衡有关，认为变应性鼻炎（AR）是以鼻腔黏膜Th2免疫反应为主的变应性炎症反应，肺气虚证是细胞因子网络自稳态动态平衡破坏的结果；在AR疾病中，Zn，Ca元素参与影响Th1/Th2平衡调节机制，提示可通过血清元素的补损，达到治疗AR的目的。 【关键词】 变应性鼻炎 微量元素 肺气虚证 动物模型 细胞因子 　　Abstract:ObjectiveAnimal model with allergic rhinitis induced on the basis of Lung Qi deficiency (LQD) pattern established earlier was prepared in gunia pigs in the way of combination with disease and pattern. Then, their serum levels of trace elements and cytokines were determined in order to investigate the influence of imbalanced Th1/Th2 cytokine network on the pathological process of experimental allergic rhinitis (EAR) in the pattern of LQD and the association of their contents and levels changes with the development of this lesion.MethodsIn the experimental studies, the animal model with allergic rhinitis induced on the basis of LQD pattern was established in guinea pigs in a way of combining a disease inducing and a pattern preparation, by using the procedures of smoke inhalation and TDI nose dropping. Then, AAS was taken to detect the serum contents of trace elements Zn, Cu and Ca and double antibody sandwitch ELISA technologies were used to measure the serum levels of IFNγ and IL4 in them. At last, a correlation analysis of trace element contents with cytokine levels was carried out to evaluate their implications in the pathogenesis of PAR.ResultsAs shown in experimental studies, the modeled animals with EAR induced on the basis of LQD pattern significantly elevated serum level of IL4, reduced serum level of IFNγ and declined ratio of IFNγ/ IL4, wit