实验性糖尿病大鼠早期视网膜电图的变化_医学论文.docVIP

实验性糖尿病大鼠早期视网膜电图的变化_医学论文 实验性糖尿病大鼠早期视网膜电图的变化_医学论文 作者：代海滨，常耀明，张作明，丁振强，李金声 【关键词】 链脲佐菌素 　　【Abstract】　AIM: To observe the changes of electroretinogram of SpragueDawley rats with earlystage (within 5 weeks) diabetes. METHODS: According to standard program recommended by International Society for Clinical Visual Electrophysiology, we recorded the rodresponse, maxresponse, oscillatory potentials (OPs), photopic electroretinograph (photERG) and 20 Hz flicker response in earlystage diabetic rats induced by streptozotocin (STZ) and agematched control ones. RESULTS: Before and at the first week after onset of diabetes, the ERG of diabetic rats did not differ significantly from that of control ones but at the second and fifth week, the implicit times of b wave of rodresponse, b wave of maxresponse, O2 wave of OPs and b wave of photERG of diabetic rats were respectively prolonged significantly compared to those of control ones. However, no significant difference was found between the ERG amplitudes of diabetic rats and those of control ones. CONCLUSION: The observations indicate that the implicit times of b wave of rodresponse, b wave of maxresponse, O2 wave of OPs and b wave of photERG are respectively prolonged significantly in earlystage diabetic rats, which can be used as indicators inspecting the early development of diabetic retinopathy. 　　【Keywords】 electroretinography diabetic retinopathy streptozotocin rats 　　【摘要】 目的： 观察实验性糖尿病早期对大鼠视网膜电图的影响. 方法： 链脲佐菌素诱导的糖尿病组大鼠和日龄相匹配的对照组正常大鼠，采用国际临床视觉电生理学会推荐的标准化方案，在糖尿病早期阶段（5 wk内）对其进行杆细胞反应、最大反应、振荡电位、明适应视网膜电图和20 Hz闪烁反应5个项目的检查. 结果： 在诱导糖尿病前和建模后1 wk时，糖尿病组大鼠与对照组相比未发现视网膜电图的异常；在建模后2 wk和5 wk时，糖尿病组杆细胞反应b波、最大反应 b波、振荡电位O2波和明适应视网膜电图b波的潜伏期都比对照组相应各波的潜伏期显著延长；两组大鼠各波的幅值始终未发现显著改变. 结论： 糖尿病大鼠在早期阶段杆细胞反应b波、最大反应b波、振荡电位O2波和明适应视网膜电图b波的潜伏期显著延长，这有可能成为监测糖尿病视网膜病变早期发展的指标. 　　【关键词】 视网膜电描记术；糖尿病视网膜病；链脲佐菌素；大鼠 　　0引言 　　糖尿病视网膜病变(diabetic retinopathy，DR)是糖尿病患者的普遍性并发症,是造成视力丧失和致盲的主要原因［1］. 然而，糖尿病导致视网膜病变的确切病理机制仍未完全阐明. 糖尿病患者视网膜微血管的异常变化通常被作为DR确诊的早期征象，这些异常变化包括毛细血管周细胞选择性丧失、基底膜增厚、微血管