尤曼桉叶总黄酮的测定方法和品质鉴别研究_药学论文.docVIP

尤曼桉叶总黄酮的测定方法和品质鉴别研究_药学论文 尤曼桉叶总黄酮的测定方法和品质鉴别研究_药学论文 【摘要】 目的研究尤曼桉叶总黄酮的测定方法和品质鉴别方法。方法用紫外分光光度法、三氯化铝比色法、硝酸铝比色法对四川引种的尤曼桉叶总黄酮进行测定并和高效液相色谱法测定结果进行比较。结果引种的尤曼桉存在叶片甲醇提取液A259/A364≈1.71 和A259/A364≈2.34的两种品质不同的植株，经液相色谱分析其芦丁含量和化学成分有明显区别。A259/A364≈1.71的植株4种方法测定结果差异不大，而A259/A364≈2.34的植株硝酸铝比色法的测定结果分别高出高效液相色谱法和三氯化铝法46.4%，39.9%。结论A259/A364≈2.34植株可能含有干扰硝酸铝比色法的成分。三氯化铝比色法测定尤曼桉叶总黄酮优于紫外分光光度法和硝酸铝法；采用热水提取、紫外分光法和高效液相色谱能很容易地将两类植株的叶片鉴别。 【关键词】 尤曼桉叶； 黄酮； 测定方法； 品质鉴别 　　Study on Determination Methods of Total Flavonoids and Quality Identification about Eucalyptus youmanii Leaves 　　Abstract：ObjectiveTo study the determination methods of total flavonoids and quality identification about Eucalyptus youmanii leaves.MethodsThe content of total flavonoids in leaves of Eucalyptus youmanii which was induced to Sichuan was detected by UV spectrophotometric method, AlCl3 colorimetric method, Al(NO3)3 colorimetric method and HPLC. Comparisons of the four methods were also discussed. ResultsThere were two types with different quality and chemical components in which the A259/A364 of extracting liquid from leaves by methanol was 1.71 and the other one was 2.34. The determination results among the four methods in leaves (A259/A364≈1.71) had no obvious difference but the results by Al(NO3)3 colorimetric method in leaves (A259/A364≈2.34) was preponderate 46.4% and 39.9% over HPLC and AlCl3 colorimetric method. ConclusionThe trees (A259/A364≈2.34) are likely to hold some components disturbing Al(NO3)3 colorimtric method. AlCl3 colorimetric method is superior to UV spectrophotometric method and Al(NO3)3 colorimetric method. The two types are easy distinguished by method of extraction with hot water, UV spectrophotometric method and HPLC. 　　Key words：Eucalyptus Youmanii leaves; Total flavonoids; Determination method; Quality identification 　　尤曼桉Eucalyptus youmanii属桃金娘科桉属单蒴盖亚属肾药组树木，四川省林科院于1997年从澳大利亚引种获得成功。其叶片富含芦丁，此外还有桉树素等黄酮类物质［1］，有较大的药用和食用开发价值。本文研究了引种的尤曼桉叶总黄酮的测定方法和品质鉴别方法，旨在为其开发利用提供参考。 　　1 材料与方法 　　1.1 仪器与试剂大连依利特高效液相色谱仪