异丙酚对在体大鼠前额叶皮质细胞外液谷氨酸和γ-氨基丁酸含量的影响_医学论文.docVIP

异丙酚对在体大鼠前额叶皮质细胞外液谷氨酸和γ-氨基丁酸含量的影响_医学论文 异丙酚对在体大鼠前额叶皮质细胞外液谷氨酸和γ-氨基丁酸含量的影响_医学论文 【摘要】 目的 观察局部灌注异丙酚对大鼠前额叶皮质细胞外液中谷氨酸和γ-氨基丁酸（GABA）含量的影响。方法 选用30只成年雄性大鼠随机分为实验组（n＝24）和对照组（n＝6），于前额叶皮质处放置微透析探头。实验组以2 μl/min灌注含不同浓度异丙酚（按照异丙酚浓度分为1、3、10、30 μmol/L 4个亚组，每组n=6）的人工脑脊液对照组灌流液中不含异丙酚。每10 min收集灌流液并应用反相高效液相色谱法测定其中谷氨酸和GABA浓度。结果 局部灌注异丙酚1 μmol/L 10 min后，各时点灌流液中谷氨酸含量与基础值比较差异无统计学意义（Pgt0.05）。异丙酚3 μmol/L局部灌注后，灌流液中谷氨酸含量于第10、20 min时降至基础值的60%（0.05），随后逐渐回升至基础值水平。前额叶皮质局部灌注异丙酚10 μmol/L 10 min后，灌流液中谷氨酸含量于第10 min至30 min显著低于基础值（0.01或lt0.05）。局部灌注异丙酚30 μmol/L 10 min后，灌流液中谷氨酸含量于第10 min至40 min均显著降低（0.01或lt0.05）。而不同浓度的异丙酚局部灌注后，各时点灌流液中GABA含量与各基础值比较差异无统计学意义（Pgt0.05）。结论 异丙酚可降低大鼠前额叶皮质细胞外液谷氨酸含量，而对GABA含量不产生影响。 【关键词】 二异丙酚 额叶前皮质 细胞外液 谷氨酸 γ-氨基丁酸（GABA） Abstract:Objective To investigate the effect of propofol locally perfused into the rat prefrontal cortex (PFC) on glutamate and GABA extracellular levels.Methods Thirty male adult rats were randomly divided into 4 groups: test groups and 1 control group (n=6 each), with a microdialysis probe inserted into the PFC. In the test groups, artificial cerebrospinal fluid (aCSF) containing different concentrations of propofol (1, 3, 10 and 30 μmol/L respectively) was perfused into the PFC at 2 μl/min in the control group, only aCSF was infused. The perfusate was collected every 10 min throughout the experiment to measure the concentrations of glutamate and GABA in the perfusate by reversed-phase high performance liquid chromatography.Results The glutamate level in the perfusate was not changed when propofol was 1 μmol/L (Pgt0.05), but it decreased significantly for 10 and 20 min when propofol was 3 μmol/L (0.05). As the concentration of propofol was further increased (to 10 and 30 μmol/L) the depression of glutamate level would extend to 40 min (0.01 or 0.05). However, the GABA level in the perfusate was not altered throughout the perfusion with different concentrations of propofol (Pgt0.05).Conclusion Propofol reduces the glutamate extracellular level in rat intact prefrontal cor