异株荨麻茎叶中黄酮类化合物含量测定方法的研究_药学论文.docVIP

异株荨麻茎叶中黄酮类化合物含量测定方法的研究_药学论文 异株荨麻茎叶中黄酮类化合物含量测定方法的研究_药学论文 作者：何斌，王兰珍，谢秀丽，刘勇 【摘要】 　　目的研究异株荨麻茎叶中黄酮类化合物的测定方法。方法分别采用Al（NO3）3络合分光光度法和HPLC法测定异株荨麻茎叶中黄酮类化合物的含量，并对测定结果进行比较；同时以黄酮类化合物的含量为指标，优选出了超声波辅助法提取异株荨麻茎叶中黄酮类化合物的最佳条件。结果超声波辅助法的最佳提取条件：体积分数为80%的乙醇溶液、料液比为1∶30，提取温度为60℃；HPLC法同时测定异株荨麻中芦丁和槲皮素的高效液相色谱条件：色谱柱选用DiamosnilTM钻石C18（250 mm×4.6 mm,5 μm），柱温25℃，流动相为甲醇：0.4%磷酸=55∶45，流速1.0 ml/min，检测波长370 nm。结论Al（NO3）3络合分光光度法操作方便，简单，快速；HPLC法可以同时准确地测定出样品中不同黄酮类化合物的含量，精确性和灵敏度高。两种方法测定的总黄酮含量相差甚微，应根据不同的测定目的，选择适当的测定方法。 【关键词】 异株荨麻 黄酮类化合物 高效液相色谱法 　　Abstract：ObjectiveTo study on determination methods of the contents of flavonoids in stems and leaves of Urtica dioica L..MethodsThe contents of flavonoids in the extracts from Urical dioica L. stems and leaves were determined and compared by Al(NO3)3 colorimetry and HPLC. The method with ultrasonic wave was applied to extract flavonoids from the leaves and stems of Urtica dioical L.. With the contents of flavonoids as the index, the best extraction conditions were selected out. Results The best extraction conditions were as follows:soaking the raw material with ethanol(80%, v/v)-water solution (1∶30) at 60℃. HPLC method was established for determination of rutin and quercetin in the sample of Urtica dioica L. Adiamonsil TM C18 (25 mm×4.6 mm,5 μm by volume) containing 0.4% phosphoric acid used as mobile phase at flow rate of 1 ml·min-1 for gradient elution. UV detector was set at 370 nm, and the column temperature was set at 25 ℃. ConclusionThe method of Al(NO3)3 colorimetry is easy and quick. The method of HPLC is accurate and sensitive. It can simultaneously and exactly determine the contents of different flavonoids. The results show that the contents of total flavonoids in Urtica dioica L. by the two methods are extremely equal. For different study objective, proper method can be chosen. 　　Key words：Urtica dioica L.； Flavonoids； HPLC 　　异株荨麻Urtica dioica L.为荨麻属Urtica linn.的一种多年生草本植物，在我国又叫大荨麻，全草或根均可入药，为一常用的民族药和民间药,广泛用于治疗风湿、尿石症、糖尿病、贫血、哮喘、心绞痛、前列腺炎、前列腺增生等［1］及一些老年性疾病