异甜菊醇对大鼠心肌缺血再灌注损伤的心电图影响_医学论文.docVIP

异甜菊醇对大鼠心肌缺血再灌注损伤的心电图影响_医学论文 异甜菊醇对大鼠心肌缺血再灌注损伤的心电图影响_医学论文 【摘要】 目的 观察异甜菊醇对大鼠心肌缺血再灌注损伤的心电图影响，并探讨它的作用与线粒体ATP敏感性钾通道(mitoKATP)开放的关系。方法 结扎大鼠冠状动脉30 min，再灌注90 min，造成心肌缺血再灌注损伤模型。结扎前静脉注射异甜菊醇和/或选择mitoKATP关闭剂5-羟基癸酸(5-HD)进行预处理，观察各组大鼠在整个缺血再灌注期间心电图ST段和室性心律失常发生情况。结果 预先给予异甜菊醇0.5、1.0和2.0mg/kg，能有效降低大鼠心肌缺血再灌注损伤引起的ST段变化和室性心律失常的发生率；但此作用可被预先给予的5-HD减弱。结论 异甜菊醇能降低心肌缺血再灌注损伤程度和室性心律失常发生率，其机理可能与mitoKATP的开放有关。 【关键词】 异甜菊醇 心肌缺血再灌注损伤 线粒体ATP敏感性钾通道 Abstract Objective To observe the effect of isostevioside on the electrocardiogram induced by ischemia and reperfusion in rat, and to explore the relationship between the cardioprotective effect of isostevioside and mitochondrial ATP- sensitive channels(mitoKATP ). Methods The myocardial injury was induced by ligation of the left coronary lasting for 30 min followed by relief for 90 min. Isosteviol (0.5, 1.0 and 2.0 mg/kg, iv) and 5-Hydroxydecanoate (10mg/kg, iv) were administrated 10～15 min prior to ischemia-reperfusion. The changes of ST segment and the incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF) were monitored during experiment. Results　The changes of ST segment and the incidence of VT and VF in rats pretreated with isosteviol were significantly less than ischemia-reperfusion group. In 5-HD+ST-1 group, the changes of ST segment and the incidence of VT and VF were less than those in isosteviol pretreated group. Conclusion Isosteviol has protective effect on myocardial injury and arrhythmia induced by ischemia-reperfusion in rats it may be mediated by mitoKATP Key words Isosteviol Myocardial ischemia-reperfusion injury Mitochondrial ATP-sensitive potassium channel 心肌缺血再灌注损伤是一个复杂的病理生理变化过程，可引起心功能衰竭、心律失常和心肌细胞死亡。目前许多的研究已证实ATP敏感性钾通道（mitochondrial ATP-sensitive potassium channel，mitoKATP）开放能有效抑制心肌缺血复灌损伤的作用。mitoKATP通道的活性受多种因素调节, 其中细胞内三磷酸腺苷（ATP）浓度是主要因素之一。在生理情况下mitoKATP通道保持关闭状态。当细胞内ATP水平下降时KATP通道趋向于开放。异甜菊醇（isosteviol, ent-16-ketobeyeran-19-oic acid）是甜菊苷的酸水解产物，研究表明：它能抑制大鼠肝细胞线粒体氧化磷酸化有关酶的活性，减少ATP的合成，有利于开放mitoKATP。且有研究发现异甜菊醇有松弛大鼠血管平滑肌作