心脑梗死患者凝血因子XIIIA基因Val34Leu多态性及血浆水平_医学论文.docVIP

心脑梗死患者凝血因子XIIIA基因Val34Leu多态性及血浆水平_医学论文 心脑梗死患者凝血因子XIIIA基因Val34Leu多态性及血浆水平_医学论文 作者：段宝民,李夏昀,逯锦涛,王卫民,李兵 【关键词】 心肌梗死 　　【Abstract】 AIM: To explore the clinical significance of the polymorphism of coagulation factor XⅢ A subunit gene (Val 34/Leu), and FXⅢ antigen and activity in plasma of patients with myocardial infarction or brain infarction. METHODS: The plasma level of FXⅢ antigen in patients and the healthy controls were measured by sandwich enzyme linked immunosorbent assay and the activity of FXⅢ was detected by Biotinlabeled assay. Allelespecific PCR was used to detect the FXⅢA subunit genotypes. RESULTS: The frequency of Leu34 genotype in patients with myocardial or brain infarction was 0.018 and 0.029 respectively, a little higher than that of controls, but no difference was found between them (Pgt0.05). There was no difference in the activity of FXⅢ between different genotypes though it was slightly higher in patients with Leu34 variant than in patients with Val 34 variant. No difference was found in FXⅢA subunit gene, FXII antigen and its activity between the controls and patients with myocardial infarction or brain infarction. CONCLUSION: There is no definitely correlation between FXⅢA gene Val34Leu polymorphism, plasma FXⅢ level and myocardial or brain infarction. 　　【Keywords】 myocardial infarction brain infarction FXⅢ Val34/Leu polymorphism FXⅢ antigen and activity 　　【摘要】 目的：探讨凝血因子FXIIIA基因Val34Leu多态性、血浆FXIII抗原及活性水平在心肌梗死与脑梗死患者中的临床意义. 方法：采用双抗体夹心ELISA法检测心肌梗死、脑梗死患者及健康对照者血浆FXIII抗原；生物素标记法检测血浆FXIII活性；等位基因特异PCR检测FXIIIA基因V34/L的基因型. 结果：心肌梗死组和脑梗死组FXIIIA基因Leu34基因频率分别为0.018和0.029，较健康人群偏高，但三组之间无显著差异（Pgt0.05），且含Leu者的FXIII活性偏高，但与Val者无差异（Pgt0.05）. 心肌梗死组和脑梗死组血浆FXIIIA亚基和B亚基抗原含量、FXIII活性与健康对照组比较无显著差异（Pgt0.05）. 结论：中国人群FXIIIA基因Val34Leu多态性、血浆FXIII抗原和活性与心肌梗死及脑梗死患病危险性无明确相关性. 　　【关键词】 心肌梗死脑梗死FXIIIA基因多态性FXIII抗原和活性 　　0引言 　　FXIIIA基因多态性与心脑血管血栓性疾病有关，其中Val34Leu引起研究者广泛的兴趣，这一多态性位点距凝血酶的酶切部位仅3个氨基酸，可影响FXIII的激活过程. 英国、芬兰和奥地利人群中Val34的存在可降低动脉闭塞性疾病如心肌梗死(MI)、脑梗死，但来自西班牙和法国南部的研究未发现有此相关性. 我们对中国汉族人群进