急性脊髓挫伤性模型的制备及观察_医学论文.docVIP

急性脊髓挫伤性模型的制备及观察_医学论文 急性脊髓挫伤性模型的制备及观察_医学论文 【摘要】 目的： 制备急性挫伤型脊髓损伤动物模型.通过观察其行为学变化和脊髓组织的病理改变,为临床治疗脊髓损伤提供理论依据. 方法： 成年雌性SD大鼠,咬除T10棘突及相应椎板,用改良的Allen法制成脊髓背侧挫伤型模型,对照组仅咬除椎板，分不同的时间行为学评分和病理检测. 结果： 对照组麻醉恢复后能站立行走,脊髓结构正常实验组行为学评分较低,脊髓损伤区域出现明显的病理改变,但随着时间的推移,可以部分的恢复. 结论： 实验组和对照组相比具有显著差异,此模型的建立为进一步了解挫伤型脊髓损伤的发病机制,研究此损伤的病理生理改变及筛选有效的预防治疗提供了良好的基础. 【关键词】 脊髓损伤；疾病模型，动物；大鼠；病理学 　　【Abstract】 AIM: To observe the functional consequence and pathological changes of spinal cord tissues in acute contusive spinal cord injury model so as to provide theoretical evidence for the clinical therapy of the impaired spinal cord. METHODS: In female adult Sprague Dawley rats, after the T10 spinous process and the corresponding vertebral lamina were removed, moderate spinal cord contusive injuries were produced by using modified Allens method in the experiment group. In control group, rats received only T10 laminectomies .The behavioral scores and the pathological alterations were recorded at different time points. RESULTS: In control group, the rats could walk after reviving, and the microstructure of spinal cord was normal.The behavioral scores of the expreriment group were lower as compared with those of the control group at different time points. The pathological alterations were very apparent in the damaged spinal cord area. When time went on, the spinal cord could be in partial functional recovery. The experimental group showed marked differences from control group. CONCLUSION: The model established in this article will contribute to understanding pathophysiological changes and screening effective therapeutic measures for this injury. 　　【Keywords】 spinal cord injuries disease models, animal rats pathology 　　0引言 　　急性脊髓损伤是一种致残率很高的的疾病. 随着交通业和建筑业的迅速发展,这样的患者呈逐年上升趋势. 脊髓损伤后的病理生理机制非常复杂,人们对此认识还很不全面及深入. 由于无法依靠临床开展系统的病理学研究,通过建立动物模型进行实验研究尤为重要. 近年来, 脊髓损伤动物模型研究是热点课题, 许多学者进行了大量的研究,以求制备可靠性高、重复性好的可检测病理变化的动物模型. 本实验我们利用Allen法制备急性挫伤型动物模型,模拟临床上外伤所致的脊髓损伤,观察其行为学变化和病理改变,为后期的脊髓搜索治疗提供实验信息. 　　1材料和方法 　　1.1材料 　　成年雌性SD大鼠40只,体质