愈邦对烧伤早期创面一氧化氮、内皮素、丙二醛的影响_药学论文.docVIP

愈邦对烧伤早期创面一氧化氮、内皮素、丙二醛的影响_药学论文 愈邦对烧伤早期创面一氧化氮、内皮素、丙二醛的影响_药学论文 作者：成春英，王建钧，汤银娟，罗成群 【摘要】 目的 探讨新型烧伤敷料“愈邦”对烧伤早期创面的可能作用机制。方法 建立大鼠深Ⅱ°烧伤模型，实验组以愈邦外用包扎，对照组以1%SDAg包扎，空白对照组不用任何药物，分别测定伤前皮肤、伤后3、6、12、24h各组创面中的NO、ET-1的含量以及伤前皮肤、伤后6、24h各组创面MDA含量，并计算各组ET-1/NO的比值。结果 实验组创面ET-1、NO、MDA含量较对照组及空白对照组均低，实验组的ET-1/NO的比值较空白对照组和对照组为低，并且较早地趋向于伤前比值。结论 愈邦烧伤敷料可能通过调节创面的ET-1、NO的含量，优化ET-1/NO比值，从而减少创面组织细胞的脂质过氧化损害程度；并且其作用优于磺胺嘧啶银。 【关键词】 愈邦；一氧化氮；内皮素-1；丙二醛 【Abstract】 Objective To study the influence of Yubang in the wound after thermal injury.Methods SD seventy-eight rats(250g～300g) of both sexes were randomly divided into 4 groups.One group(n=6) was served as nonburned control and the content of nitric oxide(NO)，endothelin-1(ET-1) and MDA in the back skin was measured after exposed to 37℃ water for 20 seconds，then the ET-1/NO was calculated.The other groups were exposed to 80℃ water for 20 seconds resulting in a deep second degree burn involving about 20% surface area.In the 2nd group，nothing was used to protect the wound and it served as blank control group.The 3rd group was used the 1% SDAg to protect the wound and served as control group.The 4th group was used the Yubang to protect the wound served as experimental group.After 3，6，12，24 hours，the content of NO，ET-1 was measured in the wound of the 2nd，3rd，4th group.After 6，24 hours the content of MDA was measured in the wound of the 2nd，3rd，4th group.In the 2nd，3rd，4th group the ET-1/NO was calculated in the 3，6，12，24 hours after thermal injury.Results The content of ET-1，NO，in the 2nd，3rd，4th group was higher than that in the 1st group and the content of MDA in the 3rd，4th was higher than that in the 1st，2nd group.The ET-1/NO in the 4th groups was lower than that in the 2nd group and 3rd groups.Conclusion The content of NO，ET-1 and MDA in the wound can increase after thermal injury，and the Yubang may be regulate the content of ET-1 and NO，and decrease the production of MDA in the wound after therapeutic injury. 【Key words】 Yubang；nitric oxide(NO)；en