星形细胞瘤中FHIT、PCNA蛋白表达及意义_医学论文.docVIP

星形细胞瘤中FHIT、PCNA蛋白表达及意义_医学论文 星形细胞瘤中FHIT、PCNA蛋白表达及意义_医学论文 作者：席峥　席焕久　 高志安　孙梅珍　王红云 【摘要】 目的 研究人脑星形细胞瘤中FHIT、PCNA蛋白的差异表达，探讨它们之间、它们与病理分级及复发之间的关系。方法 应用免疫组化SP法检测了50例星形细胞瘤中不同级别的FHIT、PCNA蛋白的表达水平，以10例非肿瘤脑组织作对照。结果 非肿瘤脑组织FHIT、PCNA蛋白阳性表达率分别为100%、0，星形细胞瘤中FHIT、PCNA蛋白阳性表达率分别为40%、86%；尽管在星形细胞瘤中FHIT、PCNA蛋白表达总体差异有显著性（0.05），Ⅱ级与Ⅲ级、Ⅱ级与Ⅳ级间比较差异有显著性（0.05），但Ⅲ级与Ⅳ级比较差异无显著性（Pgt0.05）；经统计学分析FHIT、PCNA蛋白呈显著负相关（0.01）；8例复发病例中复发前后FHIT、PCNA蛋白表达差异有显著性（0.05）。结论 星形细胞瘤中FHIT、PCNA蛋白表达之间的显著负相关，提示FHIT蛋白可能对细胞增殖具有负性调控作用。FHIT、PCNA蛋白的表达水平可能与星形细胞瘤的病理分级和复发有关，推测FHIT、PCNA蛋白的表达可能与星形细胞瘤的肿瘤侵袭性相关。 【关键词】 星形细胞瘤 FHIT蛋白 PCNA蛋白 Expression and significance of FHIT and PCNA proteins in astrocytoma　　【Abstract】 Objective To study the expression of FHIT and PCNA proteins in human brain astrocytoma To investigate the relationship between the expression of FHIT and PCNA proteins and pathological grading and recurrence in astrocytoma. Methods Immunohistochemistry staining for FHIT and PCNA proteins were performed in 50 cases of astrocytoma and 10 cases of non-tumor brain tissues. Results The total positive rate of FHIT and PCNA proteins were 100% and 0 in non-tumor brain tissues and 40% and 86% in astrocytoma respectively Though total variance of FHIT and PCNA proteins expression among tumor grades wasremarkable (0.05) , the significant differencesstatistically of FHIT and PCNA proteins were observed in gradeⅡ-grade,Ⅲand gradeⅡ-grade,Ⅳ(0.05),except those in grade Ⅲ-grade, Ⅳ(Pgt0.05) The expression of FHIT and PCNA proteins in astrocytoma were significantly negative correlated(0.01), the significant differences statistically of FHIT and PCNA proteins were observed in 8 cases of recurrence (0.05). Conclusion In astrocytoma. there are significantly inverse relations between the expression of FHIT and PCNA proteins. It is suggested that FHIT protein may play a role in the negative regulation of cell proliferation. The expression of FHIT and PCNA proteins in astrocytoma were significantly correlated with pathological grading and relapse like