枳香胶囊中挥发油提取和βCD包合工艺的研究_药学论文.docVIP

枳香胶囊中挥发油提取和β包合工艺的研究_药学论文 枳香胶囊中挥发油提取和β包合工艺的研究_药学论文 作者：王萍， 闫柏琴，张力， 李丽【摘要】 目的考察枳香胶囊中枳实、高良姜、肉桂、荜茇等挥发油的最佳提取工艺和β环糊精最佳包合工艺。方法采用单因素及L9（34）正交实验设计，以收油率作为评价提取工艺的指标优选提取工艺；以包合物收得率和挥发油包合率的综合评分作为评价包合工艺的指标优选β包合工艺。结果 挥发油最佳提取工艺为：加水10倍量，浸泡2.5 h，提取5 h；挥发油的最佳包合工艺为采用饱和水溶液法，β环糊精与挥发油的配比为8∶1，包合温度为60℃，包合时间为75 min。结论 所选提取工艺提取率高，包合工艺合理，节约成本。 【关键词】 枳香胶囊； 挥发油； β环糊精包合物； 单因素实验； 正交实验 　　Abstract：ObjectiveTo study optimum conditions for the extraction of volatile oil from Fructus Aurantii Immaturus, Rhizoma Alpiniae Officinarum, Cortex Cinnamomi and Fructus Piperis Longi in Zhixiang Capsules, and for the preparation of their βCD inclusion compounds. MethodsThe study was carried out with single factor and orthogonal design. With the percentages of extracted volatile oil of Fructus Aurantii Immaturus, Rhizoma Alpiniae Officinarum, Cortex Cinnamomi and Fructus Piperis Longi as the target markers the optimum conditions was determine for extracting the volatile oil. With the ratio of inclusion and the oiling-bearing from the inclusion as the target marker the conditions for including the oils with βCD were optimized. ResultsThe optimized extraction conditions were: the water was 10 times of the herbs in weight, the herbal medicine of Fructus Aurantii Immaturus, Rhizoma Alpiniae Officinarum, Cortex Cinnamomi and Fructus Piperis Longi should be extracted for 5 hours after advanced soaking 2.5 hours with water. The optimized inclusion conditions were: applying the saturated aqueous solution method, the proportion of oil and βCD was 1 ml∶8g, the temperature and time were at 60℃ and 75 minutes, respectively. ConclusionThe established process can be used for the extraction and the inclusion of volatile oil from Fructus Aurantii Immaturus, Rhizoma Alpiniae Officinarum, Cortex Cinnamomi and Fructus Piperis Longi in Zhixiang Capsules. The obtained ratio of oil is high, the inclusion process is suitable and the cost is economic. 　　Key words：Zhixiang Capsules; Volatile oil; βCD inclusion compounds; Single facto