树突细胞免疫对HPV感染阳性角朊细胞生物学特性影响_医学论文.docVIP

树突细胞免疫对HPV感染阳性角朊细胞生物学特性影响_医学论文 树突细胞免疫对HPV感染阳性角朊细胞生物学特性影响_医学论文 作者：严泉剑，张菊，闫小君，段杰，赵锦荣，张宇梅，苏成芝 【关键词】 树突细胞 　　关键词 :树突细胞乳头状瘤病毒，人角蛋白细胞细胞周期T淋巴细胞，细胞毒 　　摘 要:目的 观察树突细胞免疫对HPV感染阳性角朊细胞（HIPK）增殖特性的影响及诱发杀伤性T淋巴细胞（CTL）细胞毒活性的变化. 方法 用计数法绘制细胞生长曲线、流式细胞仪（FCM））检测DNA含量及细胞周期的变化，以观察HIPK增殖特性变化.同时用51 Cr释放法测定树突细胞免疫前后细胞毒性淋巴细胞（CTL）对HIPK的细胞毒作用. 结果 树突细胞免疫后，HIPK生长延缓，增殖幅度降低，倍增时间（Dt）延长，HIPK的Dt为24.7h，DCs细胞培养上清作用后HIPK的Dt为41.6h.FCM检测HIPK的G0/G1，S，G2/M各时相细胞百分比（%）分别为:57.6，28.5，13.9DCs细胞培养上清作用组分别为61.7，20.3，11.2.两者增殖指数（PI）比较差异显著（0.01）.另外没有观察到HIPK凋亡，而DCs培养上清作用组却观察6.8%的凋亡率. 　　51 Cr释放实验中，效-靶比为10∶1，5∶1，2∶1时，DCs治疗组CTL对HIPK杀伤率（%）分别为37.6，21.4，13.7而对照组为5.6，2.9，1.6. 结论 树突细胞免疫后HIPK出现了一定的增殖抑制现象，同时诱导了CTL的杀伤作用. 　　Keywords:dendritic cellspapillomavirus，humanker-atinocytecell cycleT-lymphocytes，cytotoxic 　　Abstract:AIM To investigate the characteristic change of HPV infection positive keratinocytes（HIPK）in culture after dendritic cell immunization.METHODS By comparing the difference of the growth curve with cytometre assay，DNA content and cell cycle of HIPK with flow cytometre（FCM）assay，we studied the growth characteristic distinctness of HIPK cultured with the supernatant of dendritic cell and the cytotoxicity alteration of the cytotoxic lymphocyte（CTL）to HIPK was evaluated with 51 Cr release assay.RESULTS Af-ter dendritic cell immunization，the HIPK proliferation was lagged，The double proliferation time（Dt）of HIPK was24.7h，and the Dt of HIPK treated with supernatant of DCs was41.6h.With FCM assay，the percentage（%）of the cells at distinct stage G0/G1，S，G2/M phase of HIPK was57.6，28.5，13.9while the percentage of HIPK cultured with the supertant of DCs was61.7，20.3，11.2respectively.The pro-liferation indexes of them were significantly different（0.01，χ2 test）.When effect/target ratios were10:1，5:1，2:1，the cytotoxicity rates（ 51 Cr release rate/%）of DCs treated group were37.6，21.4，13.7respectively，while those of con-trol group were5.6，2.9，1.6respectively with 51 Cr release assay.The significant differences between t