款冬花止咳有效部位和紫菀配伍“相须”部位的研究_药学论文.docVIP

款冬花止咳有效部位和紫菀配伍“相须”部位的研究_药学论文 款冬花止咳有效部位和紫菀配伍“相须”部位的研究_药学论文 作者：张巧真, 张燕, 张勉*, 黄芳, 窦昌贵, 金育 【摘要】 　　目的观察款冬花及其有效部位与紫菀不同部位配伍后对止咳作用的影响，寻找款冬花与紫菀配伍中紫菀止咳的相须部位。方法通过小鼠氨水引咳实验观察款冬花、款冬花与紫菀配伍的止咳作用。结果款冬花、款冬花与紫菀的配伍均能减少氨水引咳小鼠的咳嗽次数，其中款冬花水提物及其90％醇沉部位止咳效果最好；配伍实验显示，紫菀30％醇提物及其90％醇溶部位与款冬并配伍后，对氨水引咳小鼠的止咳作用最为明显。结论款冬花水提物的止咳效果优于乙醇提取物；水提物中90％醇沉部位效果最好，为款冬花止咳作用的有效部位。紫菀30％乙醇提取物对款冬花止咳的增效作用优于其他溶剂；而其90％醇溶部位与款冬花配伍后止咳作用的增强效果最为显著，确定该部位为配伍中紫菀的相须部位。 【关键词】 款冬花 有效部位 紫菀 配伍 相须部位 　　Abstract：ObjectiveTo search the antitussive part of Flos Farfarae (FF) and the reinforcement part of Radix Asteris (RA) in the paired drug (compound drug) of FF and RA. MethodsEvaluating the antitussive effect by cough times of mice caused by ammonia. ResultsBoth FF and the pair-drug of FF and RA can reduce the cough times of mice caused by ammonia .The aqueous extract (AE) of FF and its part of insoluble in 90% ethanol (90%insol-part) showed the best antitussive effects. The compound group of 30% ethanol extract (30%-EE) of RA with AE of FF showed obvious effect, and the groups of the part of soluble in 90% ethanol (90%sol-part) of RA with FF (AE and 90%insol-part, respectively) also showed the best antitussive effects.Conclusion The AE of FF is more effective than its ethanol extracts, and the 90%insol-part of FF is the effective antitussive part. In compound drugs, the 30%-EE and the 90%sol-part of RA can greatly reinforce the antitussive effect of FF. According to the compatibility theory of traditional Chinese medicine, the 90%sol-part of RA should be the promotional part to FF in the paired drug. 　　Key words：Flos Farfarae; Antitussive part; Radix Asteris; Compatibility; Promotional part 　　款冬花为菊科植物款冬Tussilago farfara L.的干燥花蕾，味辛、微苦，性温，归肺经，具有润肺下气、止咳化痰之功效［1］；紫菀为同科植物紫菀(Aster tataricus L. f.)的干燥根及根茎，味辛、苦，性温，归肺经，具有润肺下气、消痰止咳之功效［1］。《本草纲目》款冬花项下记载：“得紫菀良” ［2］，紫菀项下指出“款冬为之使” ［2］，《备急千金要方》、《太平圣惠方》等古代医籍中也均有款冬花与紫菀配伍使用的记载。传统配伍理论认为，紫菀长于化痰止咳，款冬花善于宣肺气而止咳，两者伍用符合相须配伍理论，是止咳化痰的常用药对，为古今众多方剂所采用。款冬花、紫菀单味药的止咳作用研究报道较多，而对两者配伍的研究未见报道。本课题组的前期研究［3］证实了该药对的相须作用，为了