格列齐特对糖尿病大鼠心肌ATP敏感性钾通道mRNA表达的影响_医学论文.docVIP

格列齐特对糖尿病大鼠心肌ATP敏感性钾通道mRNA表达的影响_医学论文 格列齐特对糖尿病大鼠心肌ATP敏感性钾通道mRNA表达的影响_医学论文 作者：　吴国亭，徐倍，李文君，程晓芸，盛春君 【关键词】 糖尿病 Effect of Gliclazide on mRNA expression of ATPsensitive potassium channels in myocardium of diabetic rats 　　【Abstract】 AIM: To observe the effect of Gliclazide on mRNA expression of ATPsensitive potassium channels（KATP） in myocardium of diabetic rats. METHODS: Rat diabetic model was made by streptozotozin ip and fed on highfat diet for 6 weeks. The rats were then allocated randomly into 3 groups: the diabetes model group, the Gliclazide group and the positive control group（treated with insulin）. A normal control group was also set up. KATPmRNA expression in myocardial cells of the rats was detected by the reverse transcriptasepolymerase chain reaction（RTPCR）. RESULTS: There was no significant difference in mRNA expression levels of KATP in heart between diabetic, insulintreated diabetic and normal control groups（Pgt0.05）. Comparison between Gliclazidetreated diabetic and nontreated diabetic rats showed that there was no change in mRNA levels of KATP in heart（Pgt0.05）. CONCLUSION: The streptozotocininduced diabetes itself does not affect the mRNA expression of KATP（SUR2 and Kir6.2） in myocardial tissues. Gliclazide with treatment dosage has no effect on gene expression of KATP. 　　【Keywords】 diabetes mellitus；myocardium；ATPsensitive potassium channels；Gliclazide 　　【摘要】 目的：观察格列齐特(Gliclazide)对糖尿病大鼠心肌组织ATP敏感性钾通道mRNA表达的影响. 方法：采用链脲佐菌素小剂量ip结合高脂饮食饲养形成2型糖尿病大鼠模型，随机分为糖尿病对照组、糖尿病胰岛素治疗组和糖尿病格列齐特组，另设正常对照组. RTPCR检测ATP敏感性钾通道mRNA表达. 结果：糖尿病组、糖尿病胰岛素治疗组和正常对照组ATP敏感性钾通道mRNA表达水平无差异（Pgt0.05）. 格列齐特治疗组ATP敏感性钾通道mRNA表达水平较正常对照组和糖尿病组无明显变化（Pgt0.05）. 结论：链脲佐菌素诱导的实验性糖尿病不影响心肌ATP敏感性钾通道mRNA表达水平；治疗剂量的格列齐特对ATP敏感性钾通道mRNA的表达无影响. 　　【关键词】 糖尿病；心肌；ATP敏感性钾通道；格列齐特 　　0引言 　　磺脲类药物（sulfonylureas，SU）治疗糖尿病尤其是对合并心血管疾病的糖尿病的安全性一直有争议［1］. SU对心血管系统产生不利影响的作用机制可能是它与心肌和血管平滑肌上ATP敏感性钾通道（ATPsensitive potassium channels，KATP）相结合，结合位点主要是该通道的调节亚基磺脲类药物受体（sulfonylurea receptor，SUR），此外内向整流钾通道6.2（inward rectifier potassium channel 6.2，