清开灵干预新生大鼠缺氧缺血脑皮质白介素-6及Fas-L蛋白的实验研究_药学论文.docVIP

清开灵干预新生大鼠缺氧缺血脑皮质白介素-6及Fas-L蛋白的实验研究_药学论文 清开灵干预新生大鼠缺氧缺血脑皮质白介素-6及Fas-L蛋白的实验研究_药学论文 作者：陈敬国，方慧云，蒋犁，程敏婷 【关键词】 脑缺氧；,,脑缺血；,,新生大鼠；,,清开灵；,,白介素-6；,,Fas-L蛋白 　　摘要：目的探讨缺氧缺血脑损伤对新生大鼠HIBD脑皮质IL-6放射免疫浓度，Fas-L蛋白表达的影响，及清开灵对新生大鼠HIBD的干预治疗作用以及有关分子机制。方法建立HIBD动物模型后分为放射免疫法测定组和免疫组化测定组，每组又分为清开灵干预组、生理盐水对照组、假手术组，根据检测需要，在缺氧缺血后24 h，3 d，7 d作IL-6脑皮质匀浆放射免疫浓度测定、Fas-L蛋白免疫组化SP法测定。结果①脑皮质组织匀浆IL-6放射免疫浓度（pg/ml）假手术组、生理盐水组、清开灵干预组：24 h依次为50.1±6.2,56.3±7.1,51.3±6.8;3 d依次为50.6±6.4,250.3±11.2,78.1±8.3;7 d依次为51.4±6.6,345.7±12.3,81.2±9.2,生理盐水组与另两组分别比较,24 hP均＞0.05,无明显变化;3,7 d生理盐水组增加明显0.05)清开灵干预组降低明显0.05)。②③④左脑皮质Fas-L蛋白表达阳性率假手术组、生理盐水组、清开灵干预组：24 h依次为8.04±3.50,120.20±6.10,21.10±5.10;3 d依次为8.20±3.10，87.6±5.90,13.40±4.20;7 d依次为8.01±2.80,8.32±2.90,8.26±2.83;生理盐水组与另两组分别比较，7 dP均＞0.05，无明显变化；24 h,3 d增多明显（0.05），清开灵干预组降低明显（0.05）。结论①新生大鼠HIBD后24 h，3 d,7 d脑皮质IL-6含量逐渐增高。②清开灵能降低新生大鼠HIBD脑皮质IL-6的产生量。③新生大鼠HIBD后，结扎侧脑皮质fas-L蛋白表达增高。④清开灵能抑制新生大鼠HIBD结扎侧脑皮质fas-L蛋白的高表达。 　　关键词：脑缺氧； 脑缺血； 新生大鼠； 清开灵； 白介素-6； Fas-L蛋白 　　Effects of QingKaiLing on the Radioimmunologic Concentrations of IL-6, the Expression of Fas-L Protein in the Cortex in Neonatal Rats after Hypoxic-ischemic Brain Damage 　　Abstract：ObjectiveTo explore the immunologic changes of interleukin-6(IL-6), the expression of fas-L protein of cortex in newborn rats after HIBD and the effects of QingKaiLing on them.MethodsTo divide animals for experiment into groups: IL-6 test group: Animals were divided into sham group, NS group and QingKailing group, IL-6 radioimmunologic concentrations of cortex were measured at 24 h, 3d and 7d after hypoxia ischemia by radioimmunoassay. Immunohistochemistry group: Animals were divided into sham group, NS group and QingKaiLing group. The changes of expression of fas-L protein were detected at 24h, 3d and 7d after hypoxia ischemia by immunohistochemistry.ResultsComparing with the sham group, the concentration of IL-6 and the expression of fas-L protein (at 24h, 3d and 7d) increased obviously in the ligated side of the brain cortex in NS group (P＜0.05 . Compari