清热祛湿法对实验性溃疡性结肠炎大鼠黏膜损伤的影响_药学论文.docVIP

清热祛湿法对实验性溃疡性结肠炎大鼠黏膜损伤的影响_药学论文 清热祛湿法对实验性溃疡性结肠炎大鼠黏膜损伤的影响_药学论文 作者：张海燕，黄穗平，黄绍刚，张向飞，王静 【摘要】 　　目的探讨清热祛湿法（肠涤清灌肠液灌肠＋肠炎清1号灌胃）对实验性溃疡性结肠炎(UC)大鼠黏膜损伤、血清和结肠组织中SOD活力及MDA含量的影响。方法成年雌性SD大鼠51只，先随机抽取8只大鼠作为正常组，不予造模；剩余的43只大鼠予造模后，第3天随机抽取2只解剖，观察造模是否成功；剩余的41只大鼠随机分为模型组11只，肠涤清组、肠涤清＋肠炎清组、柳氮磺胺吡啶(SASP)组各10只。造模后第4天开始，正常组和模型组给予生理盐水灌肠、其余三组分别给予肠涤清灌肠、肠涤清灌肠＋肠炎清灌胃、SASP灌肠＋灌胃，疗程2周。分别从大鼠的一般状况、结肠黏膜损伤指数及病理形态变化、血清和结肠组织中SOD活力及MDA含量的变化来观察清热祛湿法对2，4，6-三硝基苯磺酸诱导的实验性UC大鼠的作用机制。结果与模型组和其他两个治疗组比较，肠涤清＋肠炎清可以显著改善UC大鼠的腹泻和便血情况，减轻结肠黏膜损伤指数，改善结肠黏膜的病理形态学情况，提高血清和结肠组织中SOD活力，降低血清和结肠组织中MDA含量。结论肠涤清和肠炎清可能通过提高血清和结肠组织中SOD活力及降低MDA含量来达到对实验性UC大鼠的治疗作用。 【关键词】 清热祛湿法　溃疡性结肠炎　 肠炎清1号　肠涤清灌肠液 　　Abstract：ObjectiveTo study the effect of the method of removing heat and dispersing dampness experimental ulcerative colitis (UC) in rats.Methods8 rats were randomly selected as the normal group from 51 healthy female Sprague-Dawley (SD) rats.Active UC was induced by trinitro-benzene-sulfonic acid(TNBS) and ethanol solution in 43 rats．Three days after establishing the model of UC，the rats were randomly divided into four groups：11 in model group，10 in Changdiqing group，10 in Changdiqing + Changyanqing group and 10 in salicylazosulfapyridine (SASP) group．Four days after establishing the model of UC，the rats in both normal and model groups were treated with normal Saline enema (NSE). Rats in the other 3 groups were treated with Changdiqing enema,Changdiqing enema plus Changyanqing gavage，and SASP by gavage and enema，respectively．The course of treatment was 2 weeks and general condition， CMDI，dynamic changes of SOD activity and MDA level in serum and tissues of colon were observed．ResultsThe combination of Changdiqing and Changyanqing could significantly improve the symptoms of diarrhea and hematochezia, decrease the ulcer index of colonic mucosa,aleviate the pathological changes, increase SOD activity in serum and tissues of colon, and decrease the level of MDA in UC model rats compared with normal saline enema and the other two treatment groups.ConclusionThe