牛磺酸对钝性心脏损伤家兔心肌细胞酶的影响_医学论文.docVIP

牛磺酸对钝性心脏损伤家兔心肌细胞酶的影响_医学论文 牛磺酸对钝性心脏损伤家兔心肌细胞酶的影响_医学论文 作者：徐刚,刘日辉,刘达兴,陆红玲,周继红 【关键词】 心脏损伤 　　【Abstract】 AIM: To explore the effects of taurine on myocardial blunt injury in rabbits. METHODS: Thirty rabbits were randomly divided into 3 groups: control group, myocardial contusion group（MC group）and taurine treatment group (Tau group). BIMⅡ Horizontal Bioimpact Machine was used to make the model of myocardial contusion in MC and Tau groups. Ten minutes before and 0.5, 2, 4, 8 and 24 h after impact, blood was taken from femoral vein of the animals to measure the concentration of serum myocardial tropnion I (cTnI) and creatine phosphate kinase (CPK). At the 24th hour after impact, the myocardial tissues of all animals were taken to examine the activity of Ca2+ATPases. RESULTS: Compared with that of the control group, 2 h after myocardial contusion, the level of serum cTnI and CPK increased (0.05) and the activity of Ca2+ATPases in myocardial tissues decreased (0.01) in MC group. Compared with that of the MC group, 8 h to 24 h after impact, the concentration of serum cTnI and CPK obviously fell and the activity of Ca2+ATPases in myocardial tissues increased 1fold in Tau group. CONCLUSION: Tau can decrease the level of serum cTnI and CPK, and increase the activity of Ca2+ATPases in myocardial tissues. Tau can protect rabbits from myocardial blunt injury. 　　【Keywords 】 myocardial injury taurine cTnI CPK Ca2+ATPases 　　【摘要】 目的：探讨牛磺酸(Tau)对钝性心脏损伤家兔心肌细胞酶的影响. 方法：将健康家兔30只随机分为3组：对照组(Control)，心肌挫伤(MC)组和Tau治疗组. MC组和Tau治疗组利用BIMⅡ型水平式生物撞击机撞击兔胸前区制备钝性心脏损伤模型. 两组动物分别于撞击前10 min，撞击后0.5，2，4，8及24 h自股静脉采血检测心肌肌钙蛋白I（cardiac troponin, cTnI）与磷酸肌酸激酶（creatine phosphate kinase，CPK）；撞击24 h后各组分别取心脏检测心肌组织 Ca2+ATP酶. 对照组除不撞击胸部外，余处理均同挫伤组. 结果：与对照组相比MC组家兔撞击后2 h cTnI开始出现在血清中，且持续升高（0.05）,血清CPK含量也明显升高（0.05）；心肌Ca2+ATP酶活性明显降低（0.01）. Tau治疗组伤后8 h与24 h cTnI反应不如挫伤组强烈；CPK在伤后各时点亦明显低于挫伤组（0.05）；Ca2+ATP酶活性较挫伤组升高近1倍（0.01）. 结论: Tau能减少家兔心肌cTnI在血中的升高和CPK水平，升高心肌Ca2+ATP酶活性，对其钝性心脏损伤具有一定的保护作用. 　　【关键词】 心脏损伤；牛磺酸；心肌肌钙蛋白Ⅰ；磷酸肌酸激酶；Ca2+ATP酶