柑橘内生细菌分离鉴定及分布特性.docVIP

柑橘内生细菌分离、鉴定及分布特性 1,2，郑雪芳2，刘波2(，阮传清2，范国成2，夏育陆3，Hall D.4，段永平4 （1福建师范大学生命科学学院，福州350108；2福建省农业科学院柑橘黄龙病研究中心，福州 350003；3美国北卡州立大学有害生物综合治理研究中心, 美国 ；4美国农业部佛罗里达园艺试验室，弗罗里达34945） 摘要：对红心柚、蜜桔、金桔内生细菌进行分离鉴定并做多样性分析，为研究柑橘内生细菌与柑橘病害的生物防治奠定基础。平板分离法分离柑橘不同器官内生细菌，采用美国 MIDI微生物鉴定系统鉴定菌株，对内生细菌数量进行统计、分类，并作群落多样性分析。3种柑橘共分离得到的内生细菌属于32个种，隶属于17个属。红心柚、蜜桔、金桔分别分离得到内生细菌10个属13个种， 14个属22个种， 12个属21个种。不同种类细菌中芽孢杆菌属细菌含量最高，3种柑橘中金桔的内生细菌含量最高，不同器官中根部的内生细菌含量最高。柑橘内生细菌进行聚类分析表明，。在不同柑橘和不同器官中内生细菌的种类、数量和群落多样性存在较大差异。SIMPSON（D）指数最高SHANNON（H1）指Pielou指数本研究丰富了柑橘内生细菌资源，为柑橘内生细菌的利用提供了物质基础，并对科学说明生防作用机理具有重要价值。 关键词：柑橘 内生细菌 PLFA多样性 分布 Separation, identification and distribution of endophytic bacteria in citrus SUN Da-guang12，ZHENG Xue-fang2，LIU Bo2*, RUAN Chuan-qing2, FAN Guo-cheng2, XIA Yu-lu3, Hall D.4, DUAN Yong-ping4 1. College Of Life Science Fujian normal university, Fuzhou 350108, China; 2. Citrus Huanglongbing Research Center, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China； 3. The Center of Integrated Pest Management, North Carolina State University, North Carolina, USA； 4. Horticultural Research Laboratory (USDA/ARS), Florida, 34945, U.S.A Abstract: In order to ascertain the connection between citrus endopphytic bacteria and the Biological control about citrus Disease, the study on the citrus endophyte’s number, bacteria’s identification and community multiplicity analysis were conducted. The endophytic bacteria were isolated using the culture method and identified by Microorganism Identification System (MIDI system produced by USA). 32 microbial species identified belonging to 17 genera of bacteria were detected from endophytic bacteria in Citrus grandis Osbeckcv, Chinese honey orange and Cumquat. The content of Bacillus was the most in all endophyte, Cumquat’s endophyte was the most among 3 these citrus and the number of endophytic bacteria in root was more than other organs. The cluster analysis results showed that 32 strains could be separated into 7 groups. The community multiplicity and diversity of e