可溶性玉米须多糖的提取与纯化.pdfVIP

年第 期 中国饲料 2010 9 33 可溶性玉米须多糖的提取与纯化 安徽农业大学生命科学学院 王在贵 刘朝良 吴晓峰 方杨武 摘要 本试验采用水提醇沉法提取玉米须多糖，在不同的煎煮时间、提取温度、水料比条件下，探索最佳提取条 [ ] 件。 并用不同体积的乙醇沉淀多糖，得出最佳醇沉体积比。 水提后的玉米须残渣用 葡聚糖酶和木聚糖酶酶解提 β- 取，比较用不同的酶量酶解条件下的最适提取条件，提取的粗多糖用三氯乙酸（TCA ）法除多糖蛋白，用紫外扫描检 验蛋白是否除尽。 结果表明，玉米须多糖提取的最佳条件为：80 min、80 ℃、18 倍的水料比、2.5 倍体积乙醇沉淀效果 最佳； 浓度的 溶液除蛋白质效果最好；通过紫外扫描检测纯化样品表明糖结合蛋白基本除尽。 3 % TCA 关键词 玉米须；多糖；提取；纯化 [ ] 中图分类号 文献标识码 文章编号 （ ） [ ] S816.7 [ ] A [ ] 1004-3314 2010 09-0033-03 ， [Abstract] In this experiment the factors influencing extraction of soluble polysaccharide extraction with water from corn silk were studied and the concentration of polysaccharide was determined by Sulfuric-phenol.The factors such as ex- ， ， tracting time temperature extracting volume times of water and depositing volume times of ethanol were mainly re- ， searched.Then the polysaccharide was purifid by the method of TCA.In conclusion the opitumum extraction condition of ， ， polysaccharide from corn silk was that the extraction time was 80 min the temprature was 80 ℃ the best extracting vol- ， ume multiple of water was 18 times and the best depositing volume multiple of ethanol was 2.5 times the TCA with 3 %