受水杨酸盐浓度调控的细菌遏制系统.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, February 25; 24(2): 323-327 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究简报 王红萍, 马昱澍 , 200237 : 由于存在基因工程微生物(GEMs)不受控制地在环境中释放的风险, 利用 GEMs 的生物降解能力治理环境污染 的方法受到了限制。在大肠杆菌 JM109 中构建了一个受环境污染物调控的细菌遏制系统, 该系统是由杀伤元件和调控 元件组成的双质粒体系, 使细菌的存活受环境中水杨酸盐浓度的调控。当培养基含水杨酸盐时, 阻遏蛋白LacI 合成, 阻 止自杀基因gef 表达, 细菌快速繁殖; 当水杨酸盐不存在时, 自杀基因gef 的表达导致细胞杀伤, 菌体大量死亡。该遏制 系统可作为模型用于具有生物修复功能的基因工程菌的构建。 : 细菌遏制系统, gef , nahR , 细胞杀伤 Bacterial Containment System Regulated by the Concentra- tion of Salicylate Hongping Wang, and Yushu Ma State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China Abstract: Use of genetically engineered microorganisms (GEMs) for pollution abatement has been limited because of the risks associated with their uncontrolled release in environment. In this study, a pollutant-dependent bacterial containment system was con- structed in E. coli JM109. The system consisted of two plasmids containing a killing element and a regulatory element respectively. The survival of strains can be regulated by the concentration of salicylate in environment. In the presence of salicylate, the expression of the suicide gene gef was inhibited with the synthesis of LacI protein, leading to the normal proliferation of the strain. While in the absence of salicylate, the expression of the regulatory element was cancelled, and the expression o