四川小麦品种贮藏蛋白位点及SSR分析.pdfVIP

农业生物技术学报 Journal of Agricultural Biotechnology 2003,11 6冤院 598~604 ·研究论文· 四川小麦品种贮藏蛋白位点及SSR 分析* 春梅 傅体华** 任正隆 (四川农业大学作物遗传育种四川省重点实验室，雅安625014 ) 摘要：利用种子贮藏蛋白和SSR 标记研究了四川近20 多年育成的小麦( )品种第1 和第6 同源群染色体 的蛋白基因位点及遗传多样性。结果表明: 供试的47 个小麦品种共检测出47 条醇溶蛋白条带, 其中39 条具有多态性, 占 82.98%; 高分子量（HMW ）谷蛋白亚基共出现7 种亚基和 11 种亚 组合, 表明四川主栽小麦品种在醇溶蛋白位点上存在广泛 。 的变异, 而HMW 谷蛋白亚基遗传 础较狭窄 SSR 结果表明, 在21 个位点中有8 个(38.1%)位点具多态性, 共检测到 19 个等 位变异，供试品种在DNA 水平上存在一定的变异。聚类分析表明: 两种标记均可将供试品种(包括中国春)分为三大类。通过 Mantel 检测, 两种标记的分析结果间有显著的相关性, 反映了在蛋白质和DNA 水平上的结果可以互相补充, 增加可靠性。 关键词：小麦; 醇溶蛋白; 谷蛋白; SSR; 遗传多样性 Analyses of Storage Protein Sites and SSR in Sichuan Registered Wheat Cultivars Wang Chunmei Fu Tihua** Ren Zhenglong (Key Laboratory for Plant Genetics and Breeding of Sichuan Province, Sichuan Agricultural University, Yaan 625014, China) The genetic diversities of the first and sixth homologous chromosome groups and storage protein sites of 47 wheat ( ) cultivars registered in Sichuan were characterized by SSR markers and electrophoresis analyses. A total number of 47 gliadin bands were separated, of which 39 bands (82.99%) were polymorphic among all cultivars. Seven high-molecular-weight (HMW) glutenin subunits and 11 HMW subunit combinations were scored. These results demonstrated that broad genetic diversity of gliadin sites existed in Sichuan released wheat cultivars. However, the number of desirable HMW glutenin subunits for wheat enduse was relative poor, suggesting t