应用cDNA_AFLP技术分离应答BABA诱导的番茄抗病相关基因.pdfVIP

33 1 () Vol.33 No.1 2007 2 Journal of Hunan Agricultural University (Natural Sciences) Feb 2007 1007-1032(2007)01-0032-05 (1. 410128 2. 100081) cDNA-AFLP BABA(β-Aminobutyric Acidβ- ) 29 10 5 1 P69 2 3 Ca2+ 4 BIF15 BABA BABA cDNA-AFLP β- S436.41 A Isolation of genes induced by β-aminobutyric acid from Lycopersicon esculentum 12 1 2 1 2 1* LI Da-zhi CHEN Xiao Deng Zi-niu YANG Yu-hong XIONG Xing-yao XIE Bing-yan (1.College of Horticulture and LandscapeHNAUChangsha 410128China 2.Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China) Abstract cDNA amplified fragment length polymorphism analysis(cDNA-AFLP) was used to display transcripts whose expression is rapidly altered during the treatment of soil drench of BABA to tomato(Lycopersicon esculentum) roots Among 5 000 transcript-derived fragments23 showed increased abundance and 6 showed decreased abundanceBy cloning and sequencing10 gene fragments were obtainedBLAST search results showed that these TDFs could be divided into 5 groupsThe first group are such as pathogenesis-related proteins(PR)BIF13BIF 14 are members of a PR protein family P69Second group are transportersBIF 2BIF 3617BIF 4 have homologies to genes all responsible for transduction across membrane system of little molecular materials such as H2OCa2+ and anti-fungal agentsThe third group is signal transduction proteinsBIF5 was found to have a high homology to Ca2+-binding protein which is an important element in Ca2+ signaling pass way The forth group is molecular chaperone prot