不同产地秦艽的质量和遗传多样性研究.pdfVIP

Journal of Ch ine e M ed icinalM aterial 34 4 2011 4 # 5 17# 不同产地秦艽的质量和遗传多样性研究 吴玉泓, 吴 迪, 崔治家, 李越峰, 刘丽莎* ( 甘肃中医学院, 甘肃 兰州730000) : : , , RAPD : , , ( K) ( J) (H ) : , ; ; ; : R93115 : A : 100 1-4454( 20 11) 04-0517-03 S tudy on th e Quality and G enetic D iversity ofG en tiana ma crop hy lla Pall of D ifferen t H ab itats WU Y u-hong, WU DI, CU I Zh-i j ia, LI Yue-feng, LIU L -i ha ( Gan u College of T CM, L anzhou 730000, Ch ina) A bstrac t Objective: T o tudy the quality and genetic div er ity in Gent iana macrop hy lla of different hab itat for controlling the quality ofG entiana macrop hy lla1M ethod : M ain characteri tic and m icro cop ic identification w ere adapted to iden tify Gent iana m acro- p hy lla; H PLC method w a applied for analy i ing the content of G entiana macrop hy lla1 RAPD m ethod w a applied to tudy g enetic tructure and g enetic diver ity ofGen tiana m acrop hy lla1Re u lt: The expermi ent how ed that theG ent iana macrop hy lla of different hab-i tat have d ifferent m ain characteric tic , m icro copic identification and con tent , and effective ingredient content of gentiop icro- ide1T he lev el of popu lation genetic d iver ity of var iou group followed by HUANGZHONG County popu lation ( K ) HUAN County population( J) ZWi U M ountain popu lation( H ) 1Conclu ion: The tudy of ex ternalmorphology, internal tructure, active ingredient con- tent and g enetic level ofGent iana m acrop hy lla from d ifferent area prov ide a cientific ba i for protecting w ild pec ie and en uring the quality in introduc ing and cu ltivating Gent iana macrop hylla1 K ey w ord s D ifferent habitat ; Gent iana macrop hyl