cryIA(c)基因表达载体构建和转化甘蔗研究.pdfVIP

中国作物学会甘蔗专业委员会第十三次学术讨论会论文集(福建分册) crylA(c)基因表达载体构建与转化甘蔗研究 高世武，许莉萍，郭晋隆，徐景升，陈如凯木 (农业部甘蔗遗传改良重点实验室，中国福州350002) 摘要：根据定向克隆原则构建植物表达载体pUBCG0229，新的植物表达载体共8,602bp， 带有抗螟虫的crylA(c)基因和抗除草剂的bar基因。bar基因既可作为筛选标记基因，又可 作为甘蔗生产上有用的抗逆基因。用基因枪轰击法将pUBCG0229质粒DNA转化甘蔗品种 根筛选，移栽成活后使用Basta溶液喷洒筛选，共获得203株抗性植株，部分抗性植株通过 中。 关键词：甘蔗；螟虫；cryIA(c)基因；bar基因；基因枪 Constructionof vectorof andtransformationof expressioncrylA(c)gene sugarcane GAO Ru-kai} Shi·、Ⅳu，XU Li-ping，GUOJin-long，XUJing—sheng，CHEN LabofGenetic for Sugarcane，MinistryofAgriculture，Fuzhou350002，China) (Key Improvement Abstract：Plantvectors haveanti-insects constructed． expressionpUBCG0229，whichgene，were Thesizeof is8602 the bar bar isnot pUBCG0229bp，containingcrytA(c)andgenes．Thegene in a marker thePPTorBasta alsoafinecharacter onlyscreeninggenebyusing selection，but gene 1 transformedwiththe of the callusofGT94-19were agriculturalindustry．Theembr)romc plasmid selection、Ⅳim Basra，203 were pUBCG0229．AfterPPT(Phosphinothricin)orregeneratedplants obtained．Severaltransformedfrom of resistantwerevertifiedPCR, plants partputative plants by Dot-Southemand ofPCR results was provedcry认(c)gene blottingsequencingproducts．The imo integrated sugarcanegenome． Keyword：Sugarcane；Sugarcaneborer；cryla(c)gene；bargene；genegun 甘蔗是最主要的糖料作物，蔗糖占