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Temperature manipulating peptide self-assembly in water nanofilm.pdf

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Temperature manipulating peptide self-assembly in water nanofilm.pdf

NUCIEARSCIENCEANDTECHNIQUES25.060502(2014、 Temperaturemanipulatingpeptideself-assemblyinwaternanofilm HOUJia—Hua(侯嘉骅),DUQi—Qi—Ge(杜其其格), ZHONGRui—Bo(钟睿博),ZHANGPing(张萍),andZHANGFeng(张峰),f InnerMongoliaAgriculturalUniversity,SchoolofL Sciences,306ZhaowudaRoad,Hohhot010018,China (ReceivedAugust25,2014;acceptedinrevisedformSeptemberl3,2014;publishedonlineDecember9,2014) PeptideG 一9 sderivedfrom 3differentdiseaserelatedproteins.Theself-assemblyofG 一9 nawater nanofilm attractedmuchattentionrecently.W estudiedhow thetemperaturefactorinfluencedthepeptideself- assemblyin awaternanofilm andfound interestingphenomena:11thehigherthetemperature.thefasterthe nanofilamentsgrow;2)theGAV-9peptideofrmeddoublemonolayersinawaternanofilm at60。C.which furthersupportsthehypothesisthatthewaternanofilm couldchangethehvdroDhobicitvofmica.Webelieve theseresultscanhelpnotonlythemicrocontactprintingofamyloidpeptides,butalsoabetterunderstandingon how temperaturecontrolsthepropertiesofwaternanofilm . Keywords:Peptideself-assembly,Waternanofilm,M icrocontactprinting,Nanofilament,Hydrohpobicity DOI:1013538~.1001—8042/nst I. INTRoDUCT10N influencesGAv_9peptideself-assembling.wefabricateda depth—controllable waternanofilm system and putGA 9 Amyloiddiseases,suchasParkinsondisease,Alzheimer’s moleculesinitbyemploying Ptechnique[37,38].The disease,Creutzfeldt—Jakobdisease,TypeIIDiabetes,oftenre— resultsshow thatGAV一9adopteda “lying down”mannerto ferto insolublemisfoldedprotein f(1rmingfibrilsandtheir grow filamentsOilahydrophilic(mica)substrateatarela— depositin

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