Analysis of gene expression by real time PCR.pptVIP

Analysis of gene expression by real time PCR Analysis of real time PCR data Research Plan Today’s Laboratory Objective Output data: Amplification plot* Output data: Amplification plot Operator variability in real time PCR Relative quantitation of transcript levels* Output data: Melt curve* Identification of multiple PCR products using a melt curve Expected product melting temperatures* * * Broad and Long Term Objective To characterize the expression of the genes encoding Ribulose 1-5 bisphosphate carboxylase oxygenase-3 (RBCS3) and Chlorophyll A/B binding protein-1b (Cab-1b) in the leave