Chapter 7- Nucleic Acid Amplification Techniques.pptVIP

Chapter 7: Nucleic Acid Amplification Techniques Donna C. Sullivan, PhD Division of Infectious Diseases University of Mississippi Medical Center MOLECULAR AMPLIFICATION TECHNIQUES Nucleic acid (NA) amplification methods fall into 3 categories Target amplification systems Probe amplification systems Signal amplification Target Amplification Methods PCR – PCR using specific probes RT PCR Nested PCR-increases sensitivity, uses two sets of amplification primers, one internal to the other Multiplex PCR-two or more sets of primers specific for different targets Arbitrarily Primed PCR/Random Primer