DNA chips.pptVIP

DNA chips Technologies and utility Historical perspective DNA hybridization (1960s) Detection of hybrids hydroxyapatite radioactive labelling enzyme-linked detection fluorescent labelling Fixing sample on solid support Southern blots (1970s) Northern blots Dot blots Basic principles Main novelty is one of scale hundreds or thousands of probes rather than tens Probes are attached to solid supports Robotics are used extensively Informatics is a central component at all stages Major technologies cDNA probes ( 200 nt), usually produced by PCR, attached to either nylon or glass supports Oligonucleo