从骨碎补中制备新北美圣草苷与柚皮苷对照品的研究.pdfVIP

从骨碎补中制备新北美圣草苷和柚皮苷对照品的研究· 岳春华12，一， 李顺祥1·2” 摘要目的：建立从骨碎补中制备新北美圣草苷和柚皮苷对照品的方法。方法： LH-20柱层析和重结 结合大孔树脂富集、硅胶柱色谱、中性氧化铝吸附、Sephadex 构鉴定。结果：制备得到的新北美圣草苷和柚皮苷纯度检查符合国家药典要求，分 别为99．5％、99．3％。结论：建立的制备方法简单，对照品纯度高，可作为骨碎补药 效物质基础研究和骨碎补药材质量控制的标准对照品。 关键词骨碎补；新北美圣草苷；柚皮苷；对照品；制备 ofneoeriocitrinand referencesubstancesfrom Preparation naringin Drynariafortunei YUEChun．hual．2，3．LI Shun．xian91．2” 1．HunanProvincial Research，HunanTraditionalChinese KeyLaboratoryofDrug Academyof Medicine,Changsha 410013；2．HunanUniversity Pharmaceutical 510006 ofTCM,Changsha410007；3．Guangdong University,Guangzhou To a establish methodofneoeriocitrinand reference Abstract：Objectiveseparation naringin substancesfrom Theextractof fortuneiwasisolatedand Drynariafortunei．MethodsDrynaria purified resin，silicacolumn oxide bymacroporousgel chromatography，neutro·aluminumadsorbing，Sephadex LH一20column and structureofneoeriocitrinand was chromatographyrecrystallizations．The naringin and identifiedMS、IR、UV、1H-NMR its wasdeterminednormalization by 13C-NMR,andpurity by methodofHPLC．ResultsThecontentsofneoeriocitrinand were99．5％and99．3％ naringin The is with neoeriocitrinand method lower which respectively．Conclusiondeveloped simple cost，by callbeusedasreferencesubstancesforthe and ofChinese naringin qualitativeq