胰腺干细胞诱导分化为胰岛素分泌细胞的实验研究.pdfVIP

2007 15 3 Chin J Dia etes, M arch 2007, Vol 15, No 3 171 葛焕琦 蔡寒青 门秀丽 许世清 潘焕峰 娄晋宁 Nest in , , , mRNA, : , , 39.4% ; ; ; ; ; ; * The experimental study of pancreatic stem cells- differentiated insulin secreting cells GE H uan-qi , CA I H an-q ing , L O UJ in-ning , et al . * Dep artment of Endocrinolog , the Second H osp ital of J ilin Universit , J ilin P rovince Chang chun 130041, China Corresp onding author : L O U J in-ning , E-mail : lou. j @ mail . com Abstract Obj ective T o investigate the differentiat ion of stem cells derived from rat pancreatic tissue toward insulin secreting cells and the potential applicat ion for dia etes therapy . Methods Rat stem cells were isolated and purified f rom pancreatic duct cells using nestin conjugating magnetic eads. The stem cells w ere induced to proliferate, and differentiate towards islet-like structure in vitro. The function of these stem cell-induced islets w as further evaluated in vitro and in vivo . Re- sults The expression of insulin mRNA in the stem cells w as significantly up-regulated and the islet- like structure w as formed after proliferation and differentiation in vit ro. Immunofluoresence staining indicated that there w ere a lot of insulin positive cells and a few of glucagons positive cells in the islet- like structure. The cells released insulin in response t o glucose stimulation in vitro and hyperglycemia in dia etic animals was normalized after cell transplantation. Conclusions The stem cells derived from rat pancreas can e induced to differentiate into insulin secreting cells, w hich has a potential ap- plication for dia etes therapy. Key words Pancreas; Stem cells; Insulin; Dia etes me