土贝母皂苷提取工艺地研究.pdfVIP

′06 陕西药物分析学术研讨会 土贝母皂苷提取工艺的研究 1 2 李翠芹 ，沈志刚 (1. 陕西师范大学教育部药用植物资源与天然药物化学重点实验室，西安 710062 ； 2.西安鸿生生物技术有限公司，西安 710062) 摘要 目的：优化研究土贝母皂苷提取工艺。方法：以土贝母皂苷含量、土贝母苷甲含量和 干品得率为指标，采用正交试验法确定土贝母皂苷提取的工艺条件。结果：土贝母皂苷提取 较佳工艺为：土贝母干药材以7倍量的75%乙醇冷浸，回收乙醇后，室温下用水饱和正丁醇 萃取5次（1倍量），水洗后，浓缩到1/3体积，静置析出，＜60 ℃真空干燥。土贝母苷甲纯化 较佳工艺为：土贝母总皂苷溶于热水后（浓度20% ），用水饱和正丁醇萃取3次（溶液-正丁 醇=1 :4/3 ），合并第2 、3次萃取液，浓缩到1/2体积时，冷藏静置析出，＜60 ℃真空干燥。结 论：确定提取纯化的工艺条件简单可行，所得产品的色泽好、纯度高,此工艺具有良好的产 业化前景。 关键词：土贝母；土贝母皂苷；提取工艺 Extraction and purification of total tubeimosides from Bolbostemma paniculatum 1 2 LI Cui-qin , Shen Zhi-gang (1. Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, Shaanxi Normal University, Xi’an 710062; 2. Xi’an Honson Biotechnology Co. Ltd, Xi’an 710061) Abstract Object: To select the optimum extraction and purification process of all tubeimoisides from Bolbostemma paniculatum . Methods: The orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters. Results: The best extraction process was that the medicinal substances were marinated with seven times of ethanol-water(75 :25, V/V), after collecting ethanol, five times extraction with n-BuOH saturated with water from the solution, washed with water, concentrated n-BuOH solution to 1/3 volume, placed still to separate, dried on vacuum below 60 ℃. And the optimal purification process of Tubeimoside I was that Tubeimoside (20%) dissolved in water(60 ℃), three times extraction with n-BuOH saturated with water (solution:n-BuOH=1 :4/3). Put the seco