葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响_药学论文.docVIP

葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响_药学论文 葡萄籽原花青素对2型糖尿病大鼠肾脏功能蛋白的影响_药学论文 【摘要】 目的研究葡萄籽原花青素(grape seed procyanidin, GSPC)对2型糖尿病(type 2 diabetes mellitus, T2DM)大鼠肾脏功能蛋白的影响。方法建立T2DM大鼠模型，给予GSPC灌胃治疗12周，测定大鼠空腹血糖(FBG)、血脂(TG)、总胆固醇(TC)、超氧化物歧化酶(SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)的水平；测定胰岛素敏感指数(ISI)、肾脏肥大指数(KW/BW)和肾脏近端小管(proximal tubules, PT)钠泵活性。结果①与正常对照组比较，T2DM模型组大鼠FBG，TG，TC和KW/BW显著升高，SOD，NO，NOS，ISI和肾脏PT钠泵活性显著降低；②与T2DM模型组比较，GSPC治疗组大鼠TG，TC和KW/BW显著降低，SOD，NO，NOS，ISI和肾脏PT钠泵活性显著升高。结论 GSPC对T2DM大鼠肾脏功能蛋白具有一定的保护作用，其机制可能与GSPC降低动物体内氧化应急水平有关。 【关键词】 原花青素 2型糖尿病 氧化应急 功能蛋白 大鼠 Abstract：ObjectiveTo investigate the effect of grape seed procyanidin (GSPC) on renal functional protein of the type 2 diabetes mellitus (T2DM) rats.MethodsA model of T2DM was developed in male wistar rats. The diabetes rats were received GSPC (100 mg·kg-1·d-1) for 12 weeks. The following parameters were measured: the fasting blood sugar (FBG), blood fat (TG), total cholesterol (TC) level, KW/BW, insulin sensitivity index (ISI), superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthase (NOS) and sodium pump activity of kidney proximal tubules (PT).Results①Compared with normal control group, FBG, TG, TC level and KW/BW in the model control group were significantly increased and SOD, NO, NOS, ISI and sodium pump activity were significantly decreased; ②Compared with model control group, TG, TC level and KW/BW in the GSPC treat group were significantly decreased while SOD, NO, NOS, ISI and sodium pump activity were significantly increased. ConclusionGSPC can protect renal function in T2DM rats by decrcasing oxidative stress． Key words：Procyanidin; Type 2 diabetes mellitus; Oxidative stress; Functional protein; Rats 葡萄籽原花青素(grape seed procyanidin, GSPC)是从葡萄籽中提取出来的一类属于双黄酮衍生物的天然多酚化合物。动物实验表明，GSPC具有抗氧化、抗炎、抗动脉硬化及保护心血管等作用［1］。目前研究认为糖尿病肾病(Diabetic Nephropathy, DN)的发生除了与高血糖有关外，也与糖尿病患者抗氧化应急能力下降［2］，炎症介质增加［3］，动脉粥样硬化有密切的联系。本实验室前期研究证实了糖尿病大鼠在尿液微球蛋白出现前，肾脏功能蛋白，特别是实现肾脏功能最关键的近端小管(proximal tubule, PT)管周膜上钠泵活性已经改变［4］，所以本实验用GSPC灌胃治疗T2DM大鼠，通过检测肾脏PT钠泵活性及血清中超