降钙素基因相关肽抑制白细胞介素-1介导骨的吸收作用_医学论文.docVIP

降钙素基因相关肽抑制白细胞介素-1介导骨的吸收作用_医学论文 降钙素基因相关肽抑制白细胞介素-1介导骨的吸收作用_医学论文 作者：廉凯　杜靖远　饶振玉　罗怀灿 【关键词】 破骨细胞 关键词: 降钙素基因相关肽成骨细胞破骨细胞白细胞介素-1 摘 要:目的 了解白细胞介素-1β（IL-1β）在破骨细胞性骨吸收中的刺激作用，并评价降钙素基因相关肽（CGRP）的拮抗效果. 方法 将新生大鼠破骨细胞和成骨细胞混合培养，接种于预置象牙片的培养板中.24h后培养液中分别加入IL-1β和不同浓度的CGRP.继续培养48h，然后取出象牙片，超声处理后行甲苯胺蓝染色，光镜下观察骨吸收陷窝数目并计算其总面积. 结果 IL-1β明显增加象牙片上吸收陷窝的数目（37.2±8.2vs22.4±5.7）per slice（0.01）和面积（μm2 ，9761±2775vs4224±1381，0.01），而CGRP则能够显著抑制IL-1β的刺激作用（0.05或0.01），且抑制作用呈剂量依赖性（r1 =-0.56，0.01r2 =-0.49，0.05）. 结论 CGRP可直接作用于破骨细胞并抑制其活化，并调节成骨细胞相关因子的释放而间接影响破骨细胞功能. 　　Keywords:calcitonin gene-related peptideosteoblastosteo-clastinterleukin-1 　　Abstract:AIM To investigate the effects of interleukin-1β（IL-1β）on bone resorption in vitro，and to evaluate the inhi-bitory effect of CGRP.METHODS The osteoclasts isolated from the long bones of newborn SD rats were co-cultured with osteoblasts on ivory slices placed in24-well plates.Twenty-four hours later，conditioned media containing CGRP and/or IL-1βwere added to the wells respectively，and the culturing continued for48h.After the cells were stripped off by ultrasonication，the ivory slices were stained in toludine blue.The number of resorption lacunae on each slice was counted under light microscope and the area was measured by computer imaging analysis system.RESULTS IL-1βstimu-lated significantly bone resorption as shown by increased numbers（37.2±8.2vs22.4±5.7per slice，0.01）and areas of resorption lacunae（μm2 ，9761±2775vs4224±1381，0.01）on slices，but CGRP inhibited the effects mediated by IL-1βin a dose-dependent manner（r1 =-0.56，0.01r2 =-0.49，0.05）.CONCLUSION CGRP may inhibit osteoclastic bone resorption through a direct function on osteoclasts to block their activation，and regulate the release of cytokines by osteoblasts thus affecting indirect-ly the function of osteoclasts. 　 　　0 引言 　　破骨细胞是骨吸收过程的效应细胞，其生成、活化及其在骨吸收中的作用受细胞因子和细胞间相互作用所调节.IL-1是一种骨吸收刺激因子，可在多种骨吸收性疾病中异常地增高，其作用的方式是由成骨细胞或骨髓基质细胞介导，而非直接作用于破骨细胞.我们采用体外成骨细胞和破骨细胞联合培养的方法来观察IL-1对破骨细胞性骨吸收的刺激作用以及降钙素基因相关肽（CGR