金针菇多糖对小鼠急性肝损伤的保护作用_药学论文.docVIP

金针菇多糖对小鼠急性肝损伤的保护作用_药学论文 金针菇多糖对小鼠急性肝损伤的保护作用_药学论文 作者：李怡芳，曾怀苇，王敏，何蓉蓉，栗原博 【摘要】 目的 探讨金针菇多糖(polysaccharides of Flammulina velutipes, FVP)对CCl4诱导的急性肝损伤的保护作用。方法 小鼠腹腔注射CCl4橄榄油溶液(0.5%,5 mL·kg-1)12、24、48、72 h后测定血清ALT，确定CCl4急性肝损伤造模最佳时间为24 h。 FVP (75,150,300 mg·kg-1)连续口服给药8 d，第7天采用CCl4制备小鼠急性化学性肝损伤模型，24 h后测定FVP对血清ALT、AST活性，肝匀浆SOD活性、MDA含量的影响。结果 FVP能显著降低CCl4致急性肝损伤小鼠血清ALT、AST活性，提高肝匀浆SOD的活性,降低MDA的含量。结论 金针菇多糖对CCl4诱发的小鼠应激性肝损伤有一定的保护作用。 【关键词】 金针菇多糖(FVP);肝损伤;CCl4;ALT;AST;MDA;SOD 　　Abstract:Objective The study was designed to investigate the protective effect of polysaccharides of Flammulina velutipes (FVP) against liver injury induced by CCl4. Methods When mice were intraperitoneally injected with olive solution(0.5%,5 mL·kg-1)for 12,24,48,72 h,the plasma ALT activities were determined and the best time for CCl4induced acute liver injury in mice was 24 h. FVP (75,150 and 300 mg·kg-1) were orally administered to mice in experimental groups once per day for 8 days. On the seventh day,all mice were intraperitoneally injected with CCl4 to induce acute liver injury. 24 hours later,all mice were sacrificed,and blood and liver were collected for measurements of ALT and AST levels,the content of MDA and the activities of SOD. Results Oral administration with FVP significantly reduced the elevated levels of ALT and AST in mice with CCl4induced acute liver injury. The SOD level and the elevated content of MDA were lowered. Conclusion Polysaccharides of Flammulina velutipes had the protective effect against CCl4induced acute liver injury in mice. 　　Key words:polysaccharides of Flammulina velutipes (FVP),liver injury,CCl4,ALT,AST,MDA,SOD 　　金针菇 (Flammulina velutipes) 属担子菌亚门、金钱菌属，是一种食药兼用菌，含有多糖、糖蛋白、蛋白聚糖、朴菇素等多种生物活性物质[1]，具有较高的药用价值[2,3]。金针菇功能蛋白性蛋白诸如核糖体失活蛋白(robosome inactitating proteins,RIP)具有抗肿瘤、抗病毒、抗虫、抗真菌以及抗人免疫缺陷病毒(HIV) 等活性;真菌免疫调节蛋白(fungal immunomodulatory proteins,FIP) 具有抗癌、抗过敏、抗增殖、刺激免疫细胞产生多种细胞因子和免疫调节功能;金针菇毒素(flammutoxin,FTX) 具有引起哺乳动物红细胞裂解，使肿瘤细胞溶胀破裂，改变肠上皮细胞的渗透性，促进药物吸收等作用[4-8]。近年来，金针菇中另一种重要的生物活性物质即金针