铅中毒大鼠几种血清酶活性逆向变化的初步探讨_医学论文.docVIP

铅中毒大鼠几种血清酶活性逆向变化的初步探讨_医学论文 铅中毒大鼠几种血清酶活性逆向变化的初步探讨_医学论文 作者：李胜联,周英琼,石清峰,罗云，杨峻，欧超燕，黄玉梅，陈翔，应翔 【关键词】 铅；血清酶；逆向变化 　　Primary study on reverse changes of enzyme activities in serum of leadpoisoned rat 　　【Abstract】AIM: To understand the mechanism underlying the reverse changes of enzyme activity in serum of rat poisoned by lead. METHODS: Female Wistar rats were randomly divided into 4 groups: control group treated with distilled water lead groups treated with 10, 30 and 60 mg/kg PbAc2 , respectively,ip, once every 2 d, for 7 times. Subsequently, all animals were sacrificed for measuring the activity of ALT, AST, ALP, LDH and γGT in serum . The serum of rats treated with different concentration of PbAc2 (0, 30 and 300 μmol/L) was incubated in 37℃. Three hours later, the activities of ALT, AST, LDH, ALP and γGT in the serum were assayed. RESULTS: In vivo, compared with the control group, the activities of AST,γGT in serum were significantly higher，and the activity of ALP, LDH in serum were significantly lower (0.05), and the activities of ALT in serum were significantly lower in 10 mg/kg Pb group (0.05). In the histopathological examination, no changes in liver were found. Compared with that of the control group, in vitro, the activity of ALT in serum was significantly lower (0.05) and the activities of LDH, ALP, AST and γGT in serum showed no significant inhibition.CONCLUSION: The mechanism of the reverse changes of enzyme activity in serum is due to the different degrees of activity inhibition by lead. 　　【Keywords】lead seroenzyme, reversed changes 　　【摘要】目的： 实验研究铅中毒以阐明血清酶活性逆向变化的机制. 方法： Wistar雌性大鼠随机分为4组，对照组，对照组10， 30和60 mg/kg组. 醋酸铅腹腔注射，隔天染毒1次，7次后处死动物，取血分离血清，测血清酶活性. 另分别向大鼠血清中加入醋酸铅使其终浓度为0，30及300 μmol/L，37℃孵育3 h，测定血清酶活性. 结果： 体内实验表明，随染铅剂量的增加，血清ALP与LDH活性变化呈下降趋势（P＜0.05）；而γGT及AST活性变化呈上升趋势（与对照组比较，P＜0.05）；ALT活性变化先下降（与对照组比较，P＜0.05），而后随铅剂量的增加而呈上升趋势. 体外实验表明，铅对ALT活性抑制较明显（与对照组比较，P＜0.05），而对LDH，ALP，AST及γGT活性未见有抑制作用. 结论： 铅中毒所引起的部分血清酶活性逆向变化的机制是由于其活性受到铅等因素不同程度的抑制所致.　　【关键词】 铅；血清酶；逆向变化 　　0引言 　　当肝损害发