铜银离子协同氯化消毒对脊灰病毒核酸的破坏作用_医学论文.docVIP

铜银离子协同氯化消毒对脊灰病毒核酸的破坏作用_医学论文 铜银离子协同氯化消毒对脊灰病毒核酸的破坏作用_医学论文 作者：骆文静，张进，缪珊，侯悦 【关键词】 消毒 　　键词 :消毒大肠杆菌噬菌体脊髓灰质炎病毒 中图号:R123 文献标识码:A 　　摘 要:目的 探讨铜银离子协同氯化消毒对病毒核酸的破坏作用. 方法 铜银离子协同氯化消毒（40μg#12539L-1 银离子，400μg#12539L-1 铜离子和0.3mg#12539L-1 游离氯）前后用逆转录PCR的方法检测协同消毒前后脊灰病毒的核酸，并用免疫印记法（DIBA）检测脊灰病毒的抗原性（蛋白质）用核酸修复实验测定噬菌体f2 的核酸修复率，并用血清学方法检测噬菌体f2的抗原性. 结果 RT-PCR检测显示铜银离子协同氯化消毒前脊髓灰质炎病毒Ⅰ型（PVI）的特异条带为阳性，协同消毒后的为阴性DIBA检测显示协同消毒前后的结果均为阳性.铜银离子协同氯化消毒灭活作用后，大肠杆菌噬菌体f2 的核酸修复率随作用时间延长而降低噬菌体的抗原性与正常无明显差别. 结论 结果提示铜银离子协同氯化消毒灭活水中病毒的作用位点可能在病毒的核酸. 　　Keywords:disinfectioncoliphagepoliovirus 　　Abstract:AIM To study destructive effect of copper and silver ions in cooperation with free chlorine on nucleic acid of viruses.METHODS RNA of poliovirus was determined by using RT-PCR and the antigenicity of poliovirus was detected by using dot immunobinding assays（DIBA）before and after synergistic disinfection.The repair rate of nucleic acid of col-iphage was assayed by using nucleic acid repair experiment，and the antigenicity of coliphage was observed by using serological reaction methods.RESULTS RT-PCR showed that poliovirus I had a positive specific band before disinfec-tion，and a negative one after synergistic disinfectionDIBA showed that positive results both before and after disinfection were positive results by DIBA.The experiment on repair of nucleic acid showed that the repair rate of nucleic acid of col-iphage exposed to synergetic disinfection declined with time of action.Antigenicity of coliphage f2 showed no significant dif-ference before and after disinfection.CONCLUSION During synergistic inactivation of coliphage and poliovirus by copper and silver ions in cooperation with free chlorine，the site of their inactivation is probably nucleic acid. 　　0 引言 　　铜银离子可以增加在低浓度氯条件下对水中微生物的灭活作用［1-5］ .当用400μg#12539L -1 铜离子，40μg#12539L-1 银离子和0.3mg#12539L-1 的游离氯协同作用后对病毒灭活效果较好 ［6，7］ .但关于三者协同作用灭活病毒的作用部位是作用于病毒衣壳蛋白还是病毒的核酸尚不清楚.为进一步了解协同消毒后病毒的作用位点以及核酸的修复及变化情况，我们进行了有关观察如下. 　　1 材料和方法 　　1.1 材料 　　以大肠杆菌（285）为宿主菌，制备大肠杆菌噬菌体f