静态负荷不同强度及时间致家兔血清酶和脂质过氧化的变化_医学论文.docVIP

静态负荷不同强度及时间致家兔血清酶和脂质过氧化的变化_医学论文 静态负荷不同强度及时间致家兔血清酶和脂质过氧化的变化_医学论文 【摘要】 探讨静态负荷致骨骼肌损伤与脂质过氧化的关系。方法：雄性家兔16只分为2组，分别施加2种不同姿势静态负荷，以实验前为自身对照，观察0h、2h、4h、6h、8h血清肌酸激酶（CK）、乳酸脱氢酶（LDH）和超氧化物歧化酶(SOD)、丙二醛（MDA）含量。结果：血清CK（F＝６.00, P=0.029）、LDH活性（F=5.66，P=0.033）,Ⅱ组高于Ⅰ组，差异有统计学意义（P＜0.01），随负荷时间增加酶活力高于0h（P＜0.01）；SOD活性，2组间差异无统计学意义（P＞0.05）。组内比较,随负荷时间增加, SOD活性下降（Ⅰ组F=7.59,P=0.001；Ⅱ组F=14.23,P=0.001）不同时间SOD活性差异有统计学意义（P＜0.01）；MDA 2组间差异无统计学意义（P＞0.05）。组内比较,随负荷时间增加，MDA 增高（Ⅰ组F=9.95,P=0.000；Ⅱ组F=7.09,P=0.001），差异有统计学意义（P＜0.01）。结论：长时间静态体位，骨骼肌损伤程度与负荷持续时间、负荷强度有关，损伤原因与脂质过氧化增强和SOD减少有关。 【关键词】 静态负荷；肌酸激酶；乳酸脱氢酶；自由基；骨骼肌损伤 　　ＡｂｓｔｒａｃｔObjective:To discuss the relation of lipid peroxidation to muscle injury caused by static load.Method:Sixteen male rabbits were randomly divided into two groups, which were under two different posture exposed to static load separately. Creatine kinase(CK),lactate dehydrogenase (LDH),superoxidase(SOD), and malondialdehyde(MDA) at 2h, 4h, 6h, 8h were measured, and compared with before static load (0h) in the same group.Results:Activities of serum CK（F=6.00,P= 0.029）and LDH（F=5.66，P= 0.033）in groupⅡ were obviously higher than those in groupⅠ (P＜0.01).Compared with the levels before static load, with the prolonged static load, the serum CK and LDH for both groups increased significantly(P＜0.01).The activities of SOD in the two groups had no statistical significance (P＞0.05). Compared within the group, with the prolonged load activities of SOD in both groups（groupⅠ F=7.59,P=0.001；groupⅡF=14.23,P=0.001）,decreased significantly (P＜0.01). MDA between these two groups had no statistical significance(P＞0.05).With the prolonged load MDA increased significantly（groupⅠ F=9.95,P=0.001； groupⅡ F=7.09,P=0.001）(P＜0.05 or 0.01).Conclusion:When rabbits were exposed to posture static load, the severity of muscle injury was related to the length as well as to the strength of the static load. Enhanced lipid peroxidation may be related to the muscle injury. 　　Key wordsstatic load；creatine kinase；lactate dehydr